SibEnzyme restriction enzymes database

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Hga I

Enzyme name Hga I
Prototype HgaI
SKU SE-E461
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GACGC(N)5▼ …3'
3'… CTGCG(N)10▲ …5'
Source An E.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1007510255050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of DNA pBR322 in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on DNA pBR322
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis Incubation with > 2 units of HgaI per 1 μg of DNA and digestion > 1 hour are not recommended.
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer B
Notes Incubation with more than 2 units of enzyme per 1 μg of DNA for longer than 1 hour at 37°C is not recommended.
References
  1. Brown N.L., Smith M. Proc. Natl. Acad. Sci. U. S. A. 74: 3213-3216 (1977).Sugisaki H. Gene 3: 17-28 (1978).

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Hga I

Enzyme name Hga I
Prototype HgaI
SKU SE-E461
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GACGC(N)5▼ …3'
3'… CTGCG(N)10▲ …5'
Source An E.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1007510255050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of DNA pBR322 in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on DNA pBR322
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis Incubation with > 2 units of HgaI per 1 μg of DNA and digestion > 1 hour are not recommended.
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer B
Notes Incubation with more than 2 units of enzyme per 1 μg of DNA for longer than 1 hour at 37°C is not recommended.
References
  1. Brown N.L., Smith M. Proc. Natl. Acad. Sci. U. S. A. 74: 3213-3216 (1977).Sugisaki H. Gene 3: 17-28 (1978).