SibEnzyme restriction enzymes database

Список ферментов

HpySE526 I

Enzyme name HpySE526 I
Prototype MaeII
SKU SE-E583
Turbo version Not available
High-concentration version Not available
Recognition site
5'… A▼CGT …3'
3'… TGC▲A …5'
Source An E.coli strain, that carries the cloned HpySE 526I gene from Helicobacter pylori SE526.
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575102510050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pUC19 DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation
Supplied with enzyme 10 X SE-buffer Y
Notes

Поиск по названию

HpySE526 I

Enzyme name HpySE526 I
Prototype MaeII
SKU SE-E583
Turbo version Not available
High-concentration version Not available
Recognition site
5'… A▼CGT …3'
3'… TGC▲A …5'
Source An E.coli strain, that carries the cloned HpySE 526I gene from Helicobacter pylori SE526.
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575102510050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pUC19 DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation
Supplied with enzyme 10 X SE-buffer Y
Notes