SibEnzyme restriction enzymes database

Список ферментов

Lmn I

Enzyme name Lmn I
Prototype LmnI
SKU SE-E593
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCTCCN▼ …3'
3'… CGAG▲GN …5'
Source Lysinibacillus manganicus An22
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1007550507580
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 20 mM Tris-HCl (pH 7.5); 250 mM NaCl; 100 μg/ml BSA; 7 mM 2-mercaptoethanol, 50% glycerol. Store at -20ºC.
Ligation After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37ºC.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes

Поиск по названию

Lmn I

Enzyme name Lmn I
Prototype LmnI
SKU SE-E593
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCTCCN▼ …3'
3'… CGAG▲GN …5'
Source Lysinibacillus manganicus An22
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1007550507580
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 20 mM Tris-HCl (pH 7.5); 250 mM NaCl; 100 μg/ml BSA; 7 mM 2-mercaptoethanol, 50% glycerol. Store at -20ºC.
Ligation After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37ºC.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes