SibEnzyme restriction enzymes database

Список ферментов

Pci I

Enzyme name Pci I
Prototype BspLU11I
SKU SE-E275
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼CATGT …3'
3'… TGTAC▲A …5'
Source An E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
5075100755050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by AC m ATGT methylation. Blocked by m ACATGT methylation.
Supplied with enzyme 10 X SE-buffer O
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.
References
  1. Abdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1998). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

Поиск по названию

Pci I

Enzyme name Pci I
Prototype BspLU11I
SKU SE-E275
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼CATGT …3'
3'… TGTAC▲A …5'
Source An E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
5075100755050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by AC m ATGT methylation. Blocked by m ACATGT methylation.
Supplied with enzyme 10 X SE-buffer O
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.
References
  1. Abdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1998). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006