SibEnzyme restriction enzymes database

Список ферментов

Ple19 I

Enzyme name Ple19 I
Prototype PvuI
SKU SE-E195
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CGAT▼CG …3'
3'… GC▲TAGC …5'
Source Pseudomonas lemoignei 19
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75752525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by overlapping Dam-methylation (G m ATC) C GATC G
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).

Поиск по названию

Ple19 I

Enzyme name Ple19 I
Prototype PvuI
SKU SE-E195
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CGAT▼CG …3'
3'… GC▲TAGC …5'
Source Pseudomonas lemoignei 19
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75752525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by overlapping Dam-methylation (G m ATC) C GATC G
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).