SibEnzyme restriction enzymes database

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PspE I

Enzyme name PspE I
Prototype BstEII
SKU SE-E169
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼GTNACC …3'
3'… CCANTG▲G …5'
Source Pseudomonas species E
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10050255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes High enzyme concentration may result in star activity.
References
  1. Dedkov, V.S., Rechkunova, N.I., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994)

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PspE I

Enzyme name PspE I
Prototype BstEII
SKU SE-E169
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼GTNACC …3'
3'… CCANTG▲G …5'
Source Pseudomonas species E
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10050255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes High enzyme concentration may result in star activity.
References
  1. Dedkov, V.S., Rechkunova, N.I., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994)