SibEnzyme restriction enzymes database

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PstN I

Enzyme name PstN I
Prototype AlwNI
SKU SE-E571
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CAGNNN▼CTG …3'
3'… GTC▲NNNGAC …5'
Source Pseudomonas stutzeri 217
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25501025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme > 95% of Lambda DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes When using a buffer other than the optimal (suppied) SEBuffer, it may benecessary to add more enzyme to achieve complete digestion.

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PstN I

Enzyme name PstN I
Prototype AlwNI
SKU SE-E571
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CAGNNN▼CTG …3'
3'… GTC▲NNNGAC …5'
Source Pseudomonas stutzeri 217
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25501025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme > 95% of Lambda DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes When using a buffer other than the optimal (suppied) SEBuffer, it may benecessary to add more enzyme to achieve complete digestion.