SibEnzyme restriction enzymes database

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Set I

Enzyme name Set I
Prototype SetI
SKU SE-E537
Turbo version Not available
High-concentration version Available
Recognition site
5'… ASST▼ …3'
3'… ▲TSSA …5'
Source An E.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
Optimal buffer SE-buffer Y
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25257575100100
Unit definition One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 55°C in a total reaction volume of 20 μl.
Assayed on Double-stranded oligonucleotide
5`-CGAGTTTAT AGCT GGGCCCAAC-3`
3`-GCTCAAATA TCGA CCCGGGTTG-5`
Storage conditions 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme, approximately 50% of the pBR322 DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y [[HDR:Non-specific hydrolisis]]:
Notes Attention. SetI cleaves the canonical site as well as several additional sites with lower efficiency. Prolonged incubation may result in digestion of DNA to short oligonucleotides.
References
  1. Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease SetI // In press

Поиск по названию

Set I

Enzyme name Set I
Prototype SetI
SKU SE-E537
Turbo version Not available
High-concentration version Available
Recognition site
5'… ASST▼ …3'
3'… ▲TSSA …5'
Source An E.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
Optimal buffer SE-buffer Y
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25257575100100
Unit definition One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 55°C in a total reaction volume of 20 μl.
Assayed on Double-stranded oligonucleotide
5`-CGAGTTTAT AGCT GGGCCCAAC-3`
3`-GCTCAAATA TCGA CCCGGGTTG-5`
Storage conditions 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme, approximately 50% of the pBR322 DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y [[HDR:Non-specific hydrolisis]]:
Notes Attention. SetI cleaves the canonical site as well as several additional sites with lower efficiency. Prolonged incubation may result in digestion of DNA to short oligonucleotides.
References
  1. Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease SetI // In press