SibEnzyme restriction enzymes database

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Sfi I

Enzyme name Sfi I
Prototype SfiI
SKU SE-E123
Turbo version Available
High-concentration version Available
Recognition site
5'… GGCCNNNN▼NGGCC …3'
3'… CCGGN▲NNNNCCGG …5'
Source An E.coli strain, that carries the cloned gene Sfi I from Streptomyces fimbriatus
Optimal buffer SE-buffer G
Optimal temperature 50 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7510025252575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Blocked by overlapping Dcm methylation (C m CWGG) GG CCWGG NNGGCC. Not blocked by overlapping Dcm methylation (C m CWGG) GGCCNNNNNGG CC WGG.
Supplied with enzyme 10 X SE-buffer G, BSA
Notes Incubation at 37°C rusults in 75-100% activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Qiang, B.-Q., Schildkraut, I. Nucleic Acids Res. 12: 4507-4515 (1984). Dedkov V.S., Degtyarev S. Kh. The resctriction endonucleases detection in colonies of microorganisms Streptomyces and Nocardia // Translated from APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol 28, 1992, #. 2, 309- 313

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Sfi I

Enzyme name Sfi I
Prototype SfiI
SKU SE-E123
Turbo version Available
High-concentration version Available
Recognition site
5'… GGCCNNNN▼NGGCC …3'
3'… CCGGN▲NNNNCCGG …5'
Source An E.coli strain, that carries the cloned gene Sfi I from Streptomyces fimbriatus
Optimal buffer SE-buffer G
Optimal temperature 50 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7510025252575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Blocked by overlapping Dcm methylation (C m CWGG) GG CCWGG NNGGCC. Not blocked by overlapping Dcm methylation (C m CWGG) GGCCNNNNNGG CC WGG.
Supplied with enzyme 10 X SE-buffer G, BSA
Notes Incubation at 37°C rusults in 75-100% activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Qiang, B.-Q., Schildkraut, I. Nucleic Acids Res. 12: 4507-4515 (1984). Dedkov V.S., Degtyarev S. Kh. The resctriction endonucleases detection in colonies of microorganisms Streptomyces and Nocardia // Translated from APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol 28, 1992, #. 2, 309- 313