SibEnzyme restriction enzymes database

Список ферментов

Vne I

Enzyme name Vne I
Prototype ApaLI
SKU SE-E137
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼TGCAC …3'
3'… CACGT▲G …5'
Source An E.coli strain, that carries the cloned gene VneI from Vibrio nereis 18
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10251002525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Degtyarev, S.Kh., Rechkunova, N.I., Netesova, N.A., Tchigikov, V.E., Malygin, E.G., Kochkin, A.V., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 422-423 (1987).

Поиск по названию

Vne I

Enzyme name Vne I
Prototype ApaLI
SKU SE-E137
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼TGCAC …3'
3'… CACGT▲G …5'
Source An E.coli strain, that carries the cloned gene VneI from Vibrio nereis 18
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10251002525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Degtyarev, S.Kh., Rechkunova, N.I., Netesova, N.A., Tchigikov, V.E., Malygin, E.G., Kochkin, A.V., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 422-423 (1987).