Izoschizomers

Bst2U I

Enzyme name

Bst2U I

Prototype

BstNI

Known analogs

BptI, BseBI, BstNI, BstOI, MvaI, Ajn I, EcoRII, Psp6 I, PspGI

SKU

SE-E051

Turbo version

Not available

High-concentration version

Not available

Restriction type

Type IIPi

Recognition site

5'… CC^▼WGG …3'

3'… GGW_▲CC …5'

Source

Bacillus stearothermophilus 2U

Optimal buffer

SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Optimal temperature

60 °C

Inactivation temperature

не инактивируется

Buffer activity

B	G	O	W	Y	ROSE
75	100	50	50	10	50

Unit definition

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.

Assayed on

Lambda DNA

Storage conditions

10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.

Ligation

Nonspecific hydrolysis

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 60°C.

Methylation sensitivity

Not blocked by overlapping Dcm methylation(C m CWGG) CCWGG.

Reagents supplied with enzyme

10 X SE-buffer G, BSA

Notes

To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.

References

M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, S.Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305. 2008
M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Medical genetics, V.6, No 8, p. 29-36 (2007). (In Russian) 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Ovchinnikov bulletin of biotechnology and physical and chemical biology V.3, No 4, p. 19-27.(2007) (In Russian) 2007
M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // Ovchinnikov bulletin of biotechnology and physical and chemical biology , V.2, No 3, p. 29-38 (2006). (In Russian) 2006
V.S. Dedkov, N.A. Mikhnenkova, L.P. Vlasenko, Y.E. Tomilova, Y.G. Kashirina, D.A. Gonchar, S.Kh. Degtyarev Restriction Endonuclease AjnI from Acinetobacter johnsonii R2, an Isoschizomer of EcoRII, recognizes 5′- ↓CCWGG-3′ and cleaves dcm-methylated sites. // Biotekhnologia (Moscow), No.3, 19-24 (2004). (In Russian) 2004
Unpublished data Myakisheva, T.V., Degtyarev, S.K Unpublished observations (1995). 1995