BstSN I

Enzyme nameBstSN I
PrototypeSnaBI
Known analogsEco105I, SnaBI
SKUSE-E065
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… TAC▼GTA …3'
3'… ATG▲CAT …5'
SourceBacillus stearothermophilus SN
Optimal bufferSE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
100500105050
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onT7 DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at 20°C.
LigationAfter 5-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer B
NotesHigh enzyme concentration results in star activity
References
  1. Unpublished data Myakisheva, T.V., Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.K Unpublished observations (1995).
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