Sma I

Enzyme nameSma I
PrototypeSmaI
Known analogsCfr9I, XmaCI, Xma I
SKUSE-E177
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CCC▼GGG …3'
3'… GGG▲CCC …5'
SourceAn E.coli strain that carries the cloned Sma I gene from Serratia marcescens
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature25 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
000010050
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 25°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (HindIII-digest)
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated (by using of high concentration T4 DNA Ligase and 10% PEG) and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 25°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y.
References

No publications found.

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