Xma I

Enzyme nameXma I
PrototypeXmaI
Known analogsCfr9I, XmaCI, Sma I
SKUSE-E233
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼CCGGG …3'
3'… GGGCC▲C …5'
SourceAn E.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
75500010050
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onAdenovirus -2 DNA
Storage conditions20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
References

No publications found.

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