Source: Bacillus stearothermophilus S
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these, 95% can be recut.
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by overlapping dcm-methylation (CmCWGG):GKGCCCWGG
Blocked by GKG(5mC)MC methylation
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
|E561||S||500 u||Regular||10 000 u/ml||4,650.00 ₽|
|E562||L||2500 u||Regular||10 000 u/ml||18,600.00 ₽|