Bpu14 I

Bpu14 I

Price range: 1 900 ₽ through 7 600 ₽

Restriction endonuclease Bpu14 I

  • Recognition site:
    5'… TT▼CGAA …3'
    3'… AAGC▲TT …5'
  • Source: Bacillus pumilus 14
  • Optimal buffer: SE-buffer G
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E033 Category:

SKUРackagePackageVariantConcentrationPrice 
E033TS1000 uTurbo10 000-20 000 u/ml1 900 ₽
E034TL5000 uTurbo10 000-20 000 u/ml7 600 ₽
E033S1000 uRegular10 000-20 000 u/ml1 900 ₽
E034L5000 uRegular10 000-20 000 u/ml7 600 ₽

Description

Recognition site and hydrolysis position:

TTCGAA TT↑CGAA
AAGCTT AAGC↓TT

Source: Bacillus pumilus 14
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
50 100 25 25 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).

Additional Information

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