Description
Recognition site and hydrolysis position:
| GTSAC | ↑GTSAC |
| CASTG | CASTG↓ |
Source: An E.coli strain, that carries the cloned gene TseFI from Thermus species F35
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 100 | 50 | 0 | 25 | 50 | 50 |
Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Reagents Supplied with Enzyme:
Methylation sensitivity:
Notes:
References:
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