Ajn I

4,500.00 ₽ – 18,000.00 ₽

  • CNY: 365.94 ¥ - 1,463.76 ¥

Restriction endonuclease Ajn I

  • Recognition site: ↑CCWGG / GGWCC↓
  • Источник: Acinetobacter johnsonii R2
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 65
SKU: SE-E473 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E473S200 uRegular500-3 000 u/ml4,500.00 ₽
  • CNY: 365.94 ¥
E474L1000 uRegular500-3 000 u/ml18,000.00 ₽
  • CNY: 1,463.76 ¥

Description

Recognition site and hydrolysis position:

CCWGG ↑CCWGG
GGWCC GGWCC↓

Source: Acinetobacter johnsonii R2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
25 10 10 25 100 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by dcm methylation: CCWGGNotes: At 37°C activity is about 10% from maximum.
References:
V.S. Dedkov, N.A. Mikhnenkova, L.P. Vlasenko, J.E. Tomilova, J.G.Kashirina, D.A. Gonchar, S.Kh. Degtyarev Restriction Endonuclease AjnI from Acinetobacter johnsonii R2, an Isoschizomer of EcoRII, recognizes 5′- CCWGG-3′ and cleaves dcm-methylated sites // Translated from BIOTEHNOLOGIA (Russia) No. 3, pp. 19-24, 2004

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