Bsp13 I

Description

Recognition site and hydrolysis position:

Source: Bacillus species 13
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dam-)
Optimal SE-buffer: SE-buffer 2K
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer 2K
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dam methylation (G^mATC): TCCGGATC and GATCCGGA.
Notes:
References:
Dedkov, V.S., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations.