Bst2B I

3,565.00 14,260.00 

  • CNY: 275.61 ¥ - 1,102.44 ¥

Restriction endonuclease Bst2B I

  • Recognition site: C↑TCGTG / GAGCA↓C
  • Источник: Bacillus stearothermophilus 2B
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 60
  • Температура инактивации, 20 мин при: 80
SKU: SE-E043 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E043S200 uRegular5 000-10 000 u/ml3,565.00 
  • CNY: 275.61 ¥
E044L1000 uRegular5 000-10 000 u/ml14,260.00 
  • CNY: 1,102.44 ¥

Description

Recognition site and hydrolysis position:

CTCGTG C↑TCGTG
GAGCAC GAGCA↓C

Source: Bacillus stearothermophilus 2B
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y + BSA
Enzyme activity (%):

B G O W Y ROSE
75 25 10 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Abdurashitov, M.A., Belichenko, O.A., Degtyarev, S.K. Unpublished observations (1995).