BstAP I

6,000.00 24,000.00 

  • CNY: 463.86 ¥ - 1,855.44 ¥

Restriction endonuclease BstAP I

  • Recognition site: GCANNNN↑NTGC / CGTN↓NNNNACG
  • Источник: Bacillus stearothermophilus AP
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 60
  • Температура инактивации, 20 мин при: 80
SKU: SE-E259 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E259S200 uRegular5 000 u/ml6,000.00 
  • CNY: 463.86 ¥
E260L1000 uRegular5 000 u/ml24,000.00 
  • CNY: 1,855.44 ¥

Description

Recognition site and hydrolysis position:

GCANNNNNTGC GCANNNN↑NTGC
CGTNNNNNACG CGTN↓NNNNACG

Source: Bacillus stearothermophilus AP
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
25 25 75 100 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity. At 37°C activiti is 50% from maximum.
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References:
Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Nucleic Acids Res. 25: 2301-2302 (1997).