BstC8 I

5,060.00 20,240.00 

  • CNY: 391.19 ¥ - 1,564.75 ¥

Restriction endonuclease BstC8 I

  • Recognition site: GCN↑NGC / CGN↓NCG
  • Источник: Bacillus stearothermophilus C8
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E305 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E305S500 uRegular10 000 u/ml5,060.00 
  • CNY: 391.19 ¥
E306L2500 uRegular10 000 u/ml20,240.00 
  • CNY: 1,564.75 ¥

Description

Recognition site and hydrolysis position:

GCNNGC GCN↑NGC
CGNNCG CGN↓NCG

Source: Bacillus stearothermophilus C8
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
10 25 50 75 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by GC methylation: 5`-G(5mC)NNGC-3`/3`-CGNN(5mC)G-5`.
Notes: At 37°C activity is 50% from maximum.
References:
Abdurashitov, M.A., Dedkov, V.S., Mikhnenkova, N.A., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).
Dedkov, V.S. Novel M.BstC8I Methyltransferase Forms 5′-Gm5CNNGC-3′.Investigation of Restriction Endonuclease Sensitivity to M.BstC8I Methylation.// Mol. Gen., Microbiol. and Virology, Vol. 27. No.1. P. 40-47 (2012).