Description
| Enzyme name | SegI | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Prototype | FspAI | ||||||||||||
| Known analogs | FspAI | ||||||||||||
| SKU | SE611 | ||||||||||||
| Turbo version | Not available | ||||||||||||
| High-concentration version | Not available | ||||||||||||
| Recognition site | 5'… RTGC▼GCAY …3' 3'… YACG▲CGTR …5' | ||||||||||||
| Source | Sphingobium erigeronicola 46 | ||||||||||||
| Optimal buffer | SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) | ||||||||||||
| Optimal temperature | 37 °C | ||||||||||||
| Inactivation temperature | 65 °C | ||||||||||||
| Buffer activity |
| ||||||||||||
| Unit definition | One unit of activity is defined as the amount of enzyme required to digest 1 µg of bacteriophage T7 DNA in 50 µl of reaction mixture containing SE-buffer W at 37°C for 1 hour. | ||||||||||||
| Assayed on | Bacteriophage T7 DNA | ||||||||||||
| Storage conditions | 10 mM Tris-HCl (pH 7.6), 200 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 50% glycerol. Store at −20°C. | ||||||||||||
| Ligation | After digestion with a 3-fold excess of enzyme, DNA fragments are poorly ligated by T4 DNA ligase. Ligation efficiency exceeding 95% is achieved in the presence of 10% PEG. | ||||||||||||
| Nonspecific hydrolysis | The specific DNA digestion pattern remains unchanged after incubation of 1 µg DNA with 6 units of enzyme for 16 hours at 37°C. No nonspecific hydrolysis of single- or double-stranded oligonucleotides is observed after incubation with 3 units of enzyme for 3 hours. | ||||||||||||
| Reagents supplied with enzyme | 10× SE-buffer W | ||||||||||||
| References | No publications found. |
