Fae I

1,980.00 7,920.00 

  • CNY: 161.01 ¥ - 644.05 ¥

Restriction endonuclease Fae I

  • Recognition site: CATG↑ / ↓GTAC
  • Источник: Flavobacterium aquatile N3
  • Оптимальный буфер: SE-буфер FaeI
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E495 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E495S50 uRegular500-2 000 u/ml1,980.00 
  • CNY: 161.01 ¥
E496L250 uRegular500-2 000 u/ml7,920.00 
  • CNY: 644.05 ¥

Description

Recognition site and hydrolysis position:

CATG CATG↑
GTAC ↓GTAC

Source: Flavobacterium aquatile N3
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
pUC19 DNA
Optimal SE-buffer: SE-buffer FaeI
Enzyme activity (%):

B G O W Y ROSE
25 50 10 10 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme more then 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of pUC19 DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer FaeI, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CmATG methylation
Notes: To obtain 100% activity, BSA should be added the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S.. Unpublished observations (2006).