Description
| Recognition site | |
| Source | An E.coli strain, that carries the cloned M.HspAI gene from Haemophilus species AI. |
| Description | M.HspAI Methyltransferase modifies the internal cytosine residue (C5) in the recognition sequence forming 5`-G(5mC)GC-3`/3`-CG(5mC)G-5`. |
| Unit definition | One unit of the enzyme is the amount required to protect 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 20μl against cleavage by HspAI restriction endonuclease. |
| Reaction buffer | SE-buffer B + SAM |
| Optimal temperature | |
| Storage conditions | 10 mM Tis-HCl (pH 7.4); 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 100 mM NaCl; 50% glycerol. Store at -20°C. |
| Notes | To obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM. |
| MSDS: | Download MSDS as PDF |
