Description
Recognition site and hydrolysis position:
| GCGATCGC | GCGAT↑CGC |
| CGCTAGCG | CGC↓TAGCG |
Source: Rhizoblum galegae
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Adenovirus-2 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 75 | 50 | 10 | 25 | 100 | 100 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by overlapping Dam-methylation (GmATC): GCGATCGC
Blocked by CpG methylation.
Notes: High enzyme concentration may result in star activity.
References:
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