Rga I

2,250.00 ₽ – 9,000.00 ₽

  • CNY: 182.97 ¥ - 731.88 ¥

Restriction endonuclease Rga I

  • Recognition site: GCGAT↑CGC / CGC↓TAGCG
  • Источник: Rhizoblum galegae
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E491 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E491S200 uRegular5 000-10 000 u/ml2,250.00 ₽
  • CNY: 182.97 ¥
E492L1000 uRegular5 000-10 000 u/ml9,000.00 ₽
  • CNY: 731.88 ¥

Description

Recognition site and hydrolysis position:

GCGATCGC GCGAT↑CGC
CGCTAGCG CGC↓TAGCG

Source: Rhizoblum galegae
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Adenovirus-2 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 50 10 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by overlapping Dam-methylation (GmATC): GCGATCGC
Blocked by CpG methylation.
Notes: High enzyme concentration may result in star activity.
References:

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