SibEnzyme restriction enzymes database

Список ферментов

AccB7 I

Enzyme name AccB7 I
Prototype PflMI
SKU SE-E179
Turbo version Available
High-concentration version Not available
Recognition site
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
Source Acinetobacter calcoaceticus B7
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10100255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dcm methylation (C m CWGG) CCANNN CCTGG or CCAGG NNNTGG.
Supplied with enzyme 10 X SE-buffer G
Notes High enzyme concentration may result in star activity.
References
  1. Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Verhosina, V.A.,Vinogradova, T.P, Izv. Sib. Otd. Akad. Nauk SSSR 1: 35-37 (1990).

Поиск по названию

AccB7 I

Enzyme name AccB7 I
Prototype PflMI
SKU SE-E179
Turbo version Available
High-concentration version Not available
Recognition site
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
Source Acinetobacter calcoaceticus B7
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10100255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dcm methylation (C m CWGG) CCANNN CCTGG or CCAGG NNNTGG.
Supplied with enzyme 10 X SE-buffer G
Notes High enzyme concentration may result in star activity.
References
  1. Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Verhosina, V.A.,Vinogradova, T.P, Izv. Sib. Otd. Akad. Nauk SSSR 1: 35-37 (1990).