SibEnzyme restriction enzymes database

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Ale I

Enzyme name Ale I
Prototype OliI
SKU SE-E567
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CACNN▼NNGTG …3'
3'… GTGNN▲NNCAC …5'
Source Aureobacterium liquefaciens
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
101001010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6), 100 mM NaCl, 0.1 mM EDTA, 100 μg/ml BSA, 1 mM DTT, 50% glycerol.
Store at -20°C.
Ligation After digestion with a 3-fold excess of enzyme, more than 90% of DNA fragments can be ligated by DNA ligase, and more than 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping CpG methylation.
Supplied with enzyme 10 X SE-buffer Y.
Notes

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Ale I

Enzyme name Ale I
Prototype OliI
SKU SE-E567
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CACNN▼NNGTG …3'
3'… GTGNN▲NNCAC …5'
Source Aureobacterium liquefaciens
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
101001010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6), 100 mM NaCl, 0.1 mM EDTA, 100 μg/ml BSA, 1 mM DTT, 50% glycerol.
Store at -20°C.
Ligation After digestion with a 3-fold excess of enzyme, more than 90% of DNA fragments can be ligated by DNA ligase, and more than 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping CpG methylation.
Supplied with enzyme 10 X SE-buffer Y.
Notes