SibEnzyme restriction enzymes database

Список ферментов

Bme18 I

Enzyme name Bme18 I
Prototype AvaII
SKU SE-E029
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼GWCC …3'
3'… CCWG▲G …5'
Source Bacillus megaterium 18
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1025100751080
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Cleaved of DNA is impaired by overlapping dcm-methylation (C m CWGG) GGW CC WGG.
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Degtyarev S.K., Rechkunova N.I., Grinev A.A., Dedkov V.S. Izv. Sib. Otd. Akad. Nauk SSSR 15: 25-26 (1989). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007

Поиск по названию

Bme18 I

Enzyme name Bme18 I
Prototype AvaII
SKU SE-E029
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼GWCC …3'
3'… CCWG▲G …5'
Source Bacillus megaterium 18
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1025100751080
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Cleaved of DNA is impaired by overlapping dcm-methylation (C m CWGG) GGW CC WGG.
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Degtyarev S.K., Rechkunova N.I., Grinev A.A., Dedkov V.S. Izv. Sib. Otd. Akad. Nauk SSSR 15: 25-26 (1989). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007