SibEnzyme restriction enzymes database

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BstAU I

Enzyme name BstAU I
Prototype Bsp1407I
SKU SE-E267
Turbo version Available
High-concentration version Not available
Recognition site
5'… T▼GTACA …3'
3'… ACATG▲T …5'
Source Bacillus stearothermophillus AU
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10502510025100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme > 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W
Notes
References
  1. Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Tomilova, J.E., Degtyarev, S.K. Unpublished observations (2003)

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BstAU I

Enzyme name BstAU I
Prototype Bsp1407I
SKU SE-E267
Turbo version Available
High-concentration version Not available
Recognition site
5'… T▼GTACA …3'
3'… ACATG▲T …5'
Source Bacillus stearothermophillus AU
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10502510025100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme > 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W
Notes
References
  1. Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Tomilova, J.E., Degtyarev, S.K. Unpublished observations (2003)