SibEnzyme restriction enzymes database

Список ферментов

BstDE I

Enzyme name BstDE I
Prototype DdeI
SKU SE-E227
Turbo version Available
High-concentration version Not available
Recognition site
5'… C▼TNAG …3'
3'… GANT▲C …5'
Source Bacillus stearothermophilus DE
Optimal buffer SE-buffer G
Optimal temperature 60 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75100255010100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes At 37°C activity is 50%-75% from maximum.
References
  1. Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995). Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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BstDE I

Enzyme name BstDE I
Prototype DdeI
SKU SE-E227
Turbo version Available
High-concentration version Not available
Recognition site
5'… C▼TNAG …3'
3'… GANT▲C …5'
Source Bacillus stearothermophilus DE
Optimal buffer SE-buffer G
Optimal temperature 60 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75100255010100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes At 37°C activity is 50%-75% from maximum.
References
  1. Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995). Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006