SibEnzyme restriction enzymes database

Список ферментов

Fau I

Enzyme name Fau I
Prototype FauI
SKU SE-E209
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CCCGC(N)4▼ …3'
3'… GGGCG(N)6▲ …5'
Source An E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili
Optimal buffer SE-buffer B
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10025005050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycero; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55°C
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer B
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References
  1. Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova,N.I., Dedkov, V.S. Bioorg. Khim. 15: 130-132 (1989).

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Fau I

Enzyme name Fau I
Prototype FauI
SKU SE-E209
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CCCGC(N)4▼ …3'
3'… GGGCG(N)6▲ …5'
Source An E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili
Optimal buffer SE-buffer B
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10025005050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycero; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55°C
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer B
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References
  1. Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova,N.I., Dedkov, V.S. Bioorg. Khim. 15: 130-132 (1989).