SibEnzyme restriction enzymes database

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Pcs I

Enzyme name Pcs I
Prototype PcsI
SKU SE-E505
Turbo version Not available
High-concentration version Not available
Recognition site
5'… (5mC)GNNNNN▼NN(5mC)G …3'
3'… G(5mC)NN▲NNNNNG(5mC) …5'
Source An E.coli strain that carries the cloned Pcs I gene from Paracoccus carotinifaciens 3K.
Optimal buffer SE-buffer 3M
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50500105050
Unit definition One unit is defined as the amount of enzyme required to digest 1 µg of pMHpySE49/DriI DNA in 1 hour at 37°C in a total reaction volume of 50 µl. As a result, a linearized plasmid pMHpySE49/DriI disappears, and two DNA fragments are produced. PcsI activity assay on pMHpySE49/DriI DNA Lanes:1 and 7 - 1 Kb SE DNA-ladders 2 - Control pMHpySE49/DriI 3 - 0.25 μl Pcs I 4 - 0.5 μl Pcs I 5 - 1 μl Pcs I 6 - 2 μl Pcs I Products were separated in 1,4% TAE agarose gel.
Assayed on pMHpySE49 plasmid DNA, linearized with DriI (pMHpySE49/DriI). pMHpySE49 (3536 bp) was obtained by a
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 0.1 mg/ml BSA, 50% glycerol Store at -20°C.
Ligation
Nonspecific hydrolysis No detectable degradation of 1μg of Lambda DNA was observed after incubation with 1 units of enzyme for 16 hours at 37°C in a total reaction volume of 50 μl.
Methylation sensitivity The enzyme cleaves only C5-methylated DNA and does not cut unmodified DNA [1].
Supplied with enzyme 10 X SE-buffer 3M
Notes When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
References
  1. Chernukhin V.A., Nayakshina T.N., Tarasova M.V., Golikova L.N., Akishev A.G., Dedkov V.S., Mikhnenkova N.A., Degtyarev S.Kh. Bacterial strain Paracoccus carotinifaciens 3K- producer of PcsI site specific endonuclease. // Russian Federation patent RU 2377294 C1 (2009).

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Pcs I

Enzyme name Pcs I
Prototype PcsI
SKU SE-E505
Turbo version Not available
High-concentration version Not available
Recognition site
5'… (5mC)GNNNNN▼NN(5mC)G …3'
3'… G(5mC)NN▲NNNNNG(5mC) …5'
Source An E.coli strain that carries the cloned Pcs I gene from Paracoccus carotinifaciens 3K.
Optimal buffer SE-buffer 3M
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50500105050
Unit definition One unit is defined as the amount of enzyme required to digest 1 µg of pMHpySE49/DriI DNA in 1 hour at 37°C in a total reaction volume of 50 µl. As a result, a linearized plasmid pMHpySE49/DriI disappears, and two DNA fragments are produced. PcsI activity assay on pMHpySE49/DriI DNA Lanes:1 and 7 - 1 Kb SE DNA-ladders 2 - Control pMHpySE49/DriI 3 - 0.25 μl Pcs I 4 - 0.5 μl Pcs I 5 - 1 μl Pcs I 6 - 2 μl Pcs I Products were separated in 1,4% TAE agarose gel.
Assayed on pMHpySE49 plasmid DNA, linearized with DriI (pMHpySE49/DriI). pMHpySE49 (3536 bp) was obtained by a
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 0.1 mg/ml BSA, 50% glycerol Store at -20°C.
Ligation
Nonspecific hydrolysis No detectable degradation of 1μg of Lambda DNA was observed after incubation with 1 units of enzyme for 16 hours at 37°C in a total reaction volume of 50 μl.
Methylation sensitivity The enzyme cleaves only C5-methylated DNA and does not cut unmodified DNA [1].
Supplied with enzyme 10 X SE-buffer 3M
Notes When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
References
  1. Chernukhin V.A., Nayakshina T.N., Tarasova M.V., Golikova L.N., Akishev A.G., Dedkov V.S., Mikhnenkova N.A., Degtyarev S.Kh. Bacterial strain Paracoccus carotinifaciens 3K- producer of PcsI site specific endonuclease. // Russian Federation patent RU 2377294 C1 (2009).