SibEnzyme restriction enzymes database

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Pct I

Enzyme name Pct I
Prototype BsmI
SKU SE-E045
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GAATGCN▼ …3'
3'… CTTAC▲GN …5'
Source Planococcus citreus SM
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550100751050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme >90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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Pct I

Enzyme name Pct I
Prototype BsmI
SKU SE-E045
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GAATGCN▼ …3'
3'… CTTAC▲GN …5'
Source Planococcus citreus SM
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550100751050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme >90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006