SibEnzyme restriction enzymes database

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Psp6 I

Enzyme name Psp6 I
Prototype EcoRII
SKU SE-E453
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼CCWGG …3'
3'… GGWCC▲ …5'
Source Pseudomonas species 6
Optimal buffer SE-buffer B
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1005010257510
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dcm methylation(C m CWGG) CCWGG.
Supplied with enzyme 10 X SE-buffer B
Notes At 37°C, the activity is very low.
References
  1. Dedkov, V.S., Tomilova, J.E., Kileva, E.V., Degtyarev, S.K. Unpublished observations. (2002) V.S. Dedkov, N.A. Mikhnenkova, L.P. Vlasenko, J.E. Tomilova, J.G.Kashirina, D.A. Gonchar, S.Kh. Degtyarev Restriction Endonuclease AjnI from Acinetobacter johnsonii R2, an Isoschizomer of EcoRII, recognizes 5'- CCWGG-3' and cleaves dcm-methylated sites // Translated from BIOTEHNOLOGIA (Russia) No. 3, pp. 19-24, 2004

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Psp6 I

Enzyme name Psp6 I
Prototype EcoRII
SKU SE-E453
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼CCWGG …3'
3'… GGWCC▲ …5'
Source Pseudomonas species 6
Optimal buffer SE-buffer B
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1005010257510
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dcm methylation(C m CWGG) CCWGG.
Supplied with enzyme 10 X SE-buffer B
Notes At 37°C, the activity is very low.
References
  1. Dedkov, V.S., Tomilova, J.E., Kileva, E.V., Degtyarev, S.K. Unpublished observations. (2002) V.S. Dedkov, N.A. Mikhnenkova, L.P. Vlasenko, J.E. Tomilova, J.G.Kashirina, D.A. Gonchar, S.Kh. Degtyarev Restriction Endonuclease AjnI from Acinetobacter johnsonii R2, an Isoschizomer of EcoRII, recognizes 5'- CCWGG-3' and cleaves dcm-methylated sites // Translated from BIOTEHNOLOGIA (Russia) No. 3, pp. 19-24, 2004