SibEnzyme restriction enzymes database

Список ферментов

Sse9 I

Enzyme name Sse9 I
Prototype Tsp509I
SKU SE-E217
Turbo version Available
High-concentration version Not available
Recognition site
5'… ▼AATT …3'
3'… TTAA▲ …5'
Source An E.coli strain that carries the cloned Sse9 I gene from Sporosarcina species
Optimal buffer SE-buffer B
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1007550507575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on pBR322 DNA
Storage conditions 10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity Blocked by methylation: 5`-A(m6A)TT-3`/ 3`-TT(m6A)A-5`.
Supplied with enzyme 10 X SE-buffer B, BSA
Notes At 37°C activity is 75% from maximum. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Gonchar, D.A., Dedkov, V.S., Verkhozina, V.A., Kusner, Yu.S., Shevchenko, A.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 34: 139-141 (1998). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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Sse9 I

Enzyme name Sse9 I
Prototype Tsp509I
SKU SE-E217
Turbo version Available
High-concentration version Not available
Recognition site
5'… ▼AATT …3'
3'… TTAA▲ …5'
Source An E.coli strain that carries the cloned Sse9 I gene from Sporosarcina species
Optimal buffer SE-buffer B
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1007550507575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on pBR322 DNA
Storage conditions 10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity Blocked by methylation: 5`-A(m6A)TT-3`/ 3`-TT(m6A)A-5`.
Supplied with enzyme 10 X SE-buffer B, BSA
Notes At 37°C activity is 75% from maximum. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Gonchar, D.A., Dedkov, V.S., Verkhozina, V.A., Kusner, Yu.S., Shevchenko, A.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 34: 139-141 (1998). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006