Bsp13 I

3,960.00 ₽ – 15,840.00 ₽

  • CNY: 322.03 ¥ - 1,288.11 ¥

Restriction endonuclease Bsp13 I

  • Recognition site: T↑CCGGA / AGGCC↓T
  • Источник: Bacillus species 13
  • Оптимальный буфер: SE-буфер 2K
  • Оптимальная температура: 50
  • Температура инактивации, 20 мин при: 65
SKU: SE-E183 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E183S1000 uRegular20 000 u/ml3,960.00 ₽
  • CNY: 322.03 ¥
E184L5000 uRegular20 000 u/ml15,840.00 ₽
  • CNY: 1,288.11 ¥

Description

Recognition site and hydrolysis position:

TCCGGA T↑CCGGA
AGGCCT AGGCC↓T

Source: Bacillus species 13
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dam-)
Optimal SE-buffer: SE-buffer 2K
Enzyme activity (%):

B G O W Y ROSE
25 50 75 50 0 5

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer 2K
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dam methylation (GmATC): TCCGGATC and GATCCGGA.
Notes:
References:
Dedkov, V.S., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations.

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