BstF5 I

2,250.00 ₽ – 9,000.00 ₽

  • CNY: 182.97 ¥ - 731.88 ¥

Restriction endonuclease BstF5 I

  • Recognition site: GGATGNN↑ / CCTAC↓NN
  • Источник: Bacillus stearothermophilus F5
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: 80
SKU: SE-E031 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E031S500 uRegular10 000 u/ml2,250.00 ₽
  • CNY: 182.97 ¥
E032L2500 uRegular10 000 u/ml9,000.00 ₽
  • CNY: 731.88 ¥

Description

Recognition site and hydrolysis position:

GGATGNN GGATGNN↑
CCTACNN CCTAC↓NN

Source: Bacillus stearothermophilus F5
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 50 25 50 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
References:
Abdurashitov, M.A., Kileva, E.V., Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Gene 172: 49-51 (1996).

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