Mhl I

5,000.00 20,000.00 

  • CNY: 406.60 ¥ - 1,626.40 ¥

Restriction endonuclease Mhl I

  • Recognition site: GDGCH↑C / C↓HCGDG
  • Источник: Micrococcus halobius SD
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E049 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E049S500 uRegular5 000-10 000 u/ml5,000.00 
  • CNY: 406.60 ¥
E050L2500 uRegular5 000-10 000 u/ml20,000.00 
  • CNY: 1,626.40 ¥

Description

Recognition site and hydrolysis position:

GDGCHC GDGCH↑C
CHCGDG C↓HCGDG

Source: Micrococcus halobius SD
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
10 25 75 100 10 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Shapovalova, M.A., Abdurashitov, M.A., Dedkov, V.S., Kashirina, J.G., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000).