Pst I

Pst I

Price range: 2 800 ₽ through 11 200 ₽

Restriction endonuclease Pst I

  • Recognition site:
    5'… CTGCA▼G …3'
    3'… G▲ACGTC …5'
  • Source: An E.coli strain that carries the cloned Pst I gene from Providencia stuartii
  • Optimal buffer: SE-buffer O
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 80 °C
SKU: SE-E109 Category:

SKUРackagePackageVariantConcentrationPrice 
E109S4000 е.а.Regular20000 е.а./мл2 800 ₽
E110L20000 е.а.Regular20000 е.а./мл11 200 ₽
E109TS4000 е.а.Turbo20000 е.а./мл2 800 ₽
E110TL20000 е.а.Turbo20000 е.а./мл11 200 ₽
E109XS4000 е.а.Concentrated50000 е.а./мл2 800 ₽
E110XL20000 е.а.Concentrated50000 е.а./мл11 200 ₽

Description

Recognition site and hydrolysis position:

CTGCAG CTGCA↑G
GACGTC G↓ACGTC

Source: An E.coli strain that carries the cloned Pst I gene from Providencia stuartii
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
10 25 100 25 25 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O, BSA (except E109T and E110T).
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Smith, D.I., Blattner, F.R., Davies, J. Nucleic Acids Res. 3: 343-353 (1976).

Additional Information

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