Эндонуклеазы рестрикции

Описание

Эндонуклеазы рестрикции – сайт-специфические дезоксирибонуклеазы , взаимодействующие со строго определенными короткими нуклеотидными последовательностями двухцепочечной ДНК и расщепляющие фосфодиэфирные связи обеих цепей в определенном месте участка узнавания или рядом с ним

Доступны в нескольких вариантах:

  • Regular – традиционные;
  • Turbo – сертифицированы для быстрого гидролиза 5-15 минут,
  • Concentrated – высокоактивные или высококонцентрированные варианты ферментов

Найти изошизомеры наших ферментов от других производителей вы можете на вкладке Изошизомеры

На вкладке Активность в буферах приведены оптимальные буферы для каждого фермента, оптимальные температуры, а также активность в каждом из буферов SibEnzyme.

Информация о блокировании активности рестриктаз при полном или частичном перекрывании их сайтов узнавания метилированием находится на вкладке Чувствительность к метилированию

Ферменты

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Активность в буферах

Активность эндонуклеаз рестрикции в различных SE-буферах и температура инактивации за 20 минут

Фермент Сайт узнавания Оптим. буфер BSA Активность (в % от максимальной) Оптимальная температура, °С Инактивация, °С
B G O W Y Rose
Aat II
GACGT↑C
C↓TGCAG
 Y – 10 – 25 25 – 50 10 – 25 25 – 50 100 50 37 65
Abs I
CC↑TCGAGG
GGAGCT↓CC
 * – 75 – 100 10 – 25 0 – 10 50 – 75 0 – 10 50 37 65
Acc16 I
TGC↑GCA
ACG↓CGT
 W – 50 – 75 75 – 100 25 – 50 100 75 – 100 70 37 65
Acc36 I
ACCTGC(N)4↑
TGGACG(N)8↓
 Y – 25 – 50 25 – 50 50 – 75 50 – 75 100 100 37 65
Acc65 I
G↑GTACC
CCATG↓G
 W – 10 – 25 25 – 50 75 – 100 100 10 – 25 100 37 65
AccB1 I
G↑GYRCC
CCRYG↓G
 K + 50 – 75 10 – 25 10 – 25 75 – 100 50 – 75 30 37 65
AccB7 I
CCANNNN↑NTGG
GGTN↓NNNNACC
 G – 10 – 25 100 25 – 50 50 – 75 50 – 75 100 37 65
AccBS I
GAG↑CGG
CTC↓GCC
 Y – 75 – 100 75 – 100 25 – 50 25 – 50 100 100 37 65
Acl I
AA↑CGTT
TTGC↓AA
 Y + 0 – 10 0 – 10 0 – 10 0 – 10 100 80 37 65
AclW I
GGATC(N)4↑
CCTAG(N)5↓
 Y + 75 – 100 50 – 75 0 – 10 0 – 10 100 30 37 65
Aco I
Y↑GGCCR
RCCGG↓Y
 Y – 50 – 75 75 – 100 50 – 75 25 – 50 100 100 37 65
Acs I
R↑AATTY
YTTAA↓R
 W + 25 – 50 50 – 75 50 – 75 100 10 – 25 100 50 80
Acu I
CTGAAG(N)16↑
GACTTC(N)14↓
 Y+ SAM + 25 – 50 50 – 75 50 – 75 75 – 100 100 50 37 65
Afe I
AGC↑GCT
TCG↓CGA
 Y – 10 – 25 25 – 50 75 – 100 75 – 100 100 100 37 65
Ags I
TTS↑AA
AA↓STT
 Y + 75 – 100 50 – 75 10 – 25 10 – 25 100 50 37 65
Ahl I
A↑CTAGT
TGATC↓A
 B + 100 75 – 100 25 – 50 25 – 50 75 – 100 100 37 N
Ajn I
↑CCWGG
GGWCC↓
 Y – 25 – 50 10 – 25 10 – 25 25 – 50 100 50 55 65
Alu I
AG↑CT
TC↓GA
 Y – 75 – 100 75 – 100 10 – 25 50 – 75 100 50 37 65
AluB I
AG↑CT
TC↓GA
 B + 100 75 – 100 10 – 25 10 – 25 75 – 100 60 37 65
Ama87 I
C↑YCGRG
GRGCY↓C
 W + 10 – 25 50 – 75 75 – 100 100 0 – 10 25 37 65
Apa I
GGGCC↑C
C↓CCGGG
 Y + 50 – 75 25 – 50 0 – 10 0 – 10 100 50 37 65
Ars I
↑(N)8GACNNNNNNTTYG(N)11↑
↓(N)13CTGNNNNNNAARC(N)6↓
 Y + 0 – 10 0 – 10 0 – 10 0 – 10 100 20 30 65
AsiG I
A↑CCGGT
TGGCC↓A
 O – 10 – 25 25 – 50 100 75 – 100 10 – 25 75 37 65
AsiS I
GCGAT↑CGC
CGC↓TAGCG
 B – 100 75 – 100 0 – 10 10 – 25 25 – 50 25 37 80
AspA2 I
C↑CTAGG
GGATC↓C
 W + 10 – 25 50 – 75 75 – 100 100 75 – 100 10 37 80
AspLE I
GCG↑C
C↓GCG
 O – 10 – 25 75 – 100 100 50 – 75 25 – 50 100 37 N
AspS9 I
G↑GNCC
CCNG↓G
 W – 50 – 75 50 – 75 75 – 100 100 50 – 75 75 37 65
AsuC2 I
CC↑SGG
GGS↓CC
 Y – 75 – 100 50 – 75 10 – 25 25 – 50 100 40 37 65
AsuHP I
GGTGA(N)8↑
CCACT(N)7↓
 O – 10 – 25 50 – 75 100 75 – 100 25 – 50 100 37 65
AsuNH I
G↑CTAGC
CGATC↓G
 Y + 75 – 100 50 – 75 0 – 10 0 – 10 100 25 37 65
BamH I
G↑GATCC
CCTAG↓G
 G + 25 – 50 100 75 – 100 75 – 100 25 – 50 100 37 65
Bar I
↑(N)7GAAGNNNNNNTAC(N)12↑
↓(N)12CTTCNNNNNNATG(N)7↓
 * – 0 – 10 0 – 10 25 – 50 50 – 75 10 – 25 40 37 65
Bbv12 I
GWGCW↑C
C↓WCGWG
 O – 0 – 10 10 – 25 100 75 – 100 10 – 25 60 37 80
Bgl I
GCCNNNN↑NGGC
CGGN↓NNNNCCG
 2W – 50 – 75 50 – 75 0 – 10 75 – 100 25 – 50 100 37 65
Bgl II
A↑GATCT
TCTAG↓A
 O – 0 – 10 10 – 25 100 25 – 50 10 – 25 100 37 80
Bme18 I
G↑GWCC
CCWG↓G
 O – 10 – 25 25 – 50 100 75 – 100 10 – 25 80 37 65
Bmt I
GCTAG↑C
C↓GATCG
 W – 10 – 25 50 – 75 50 – 75 100 75 – 100 100 37 65
Bmu I
ACTGGG(N)5↑
TGACCC(N)4↓
 Y – 75 – 100 75 – 100 25 – 50 10 – 25 100 25 37 65
Bpm I
CTGGAG(N)16↑
GACCTC(N)14↓
 W + 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
Bpu10 I
CC↑TNAGC
GGANT↓CG
 K – 10 – 25 25 – 50 50 – 75 50 – 75 25 – 50 100 37 80
Bpu14 I
TT↑CGAA
AAGC↓TT
 G – 50 – 75 100 25 – 50 25 – 50 75 – 100 100 37 65
Bsa29 I
AT↑CGAT
TAGC↓TA
 G + 25 – 50 100 50 – 75 50 – 75 75 – 100 100 37 65
Bsc4 I
CCNNNNN↑NNGG
GGNN↓NNNNNCC
 W + 75 – 100 75 – 100 50 – 75 100 25 – 50 90 55 80
Bse1 I
ACTGGN↑
TGAC↓CN
 Y – 75 – 100 75 – 100 25 – 50 10 – 25 100 100 65 80
Bse118 I
R↑CCGGY
YGGCC↓R
 O – 0 – 10 50 – 75 100 75 – 100 25 – 50 100 65 80
Bse21 I
CC↑TNAGG
GGANT↓CC
 Y – 50 – 75 50 – 75 10 – 25 25 – 50 100 40 37 80
Bse3D I
GCAATGNN↑
CGTTAC↓NN
 G – 10 – 25 100 25 – 50 50 – 75 75 – 100 100 60 80
Bse8 I
GATNN↑NNATC
CTANN↓NNTAG
 G – 25 – 50 100 75 – 100 75 – 100 50 – 75 100 60 80
BseP I
G↑CGCGC
CGCGC↓G
 G – 50 – 75 100 75 – 100 50 – 75 50 – 75 100 50 65
BseX3 I
C↑GGCCG
GCCGG↓C
 O – 10 – 25 25 – 50 100 50 – 75 10 – 25 25 50 80
BslF I
GGGAC(N)10↑
CCCTG(N)14↓
 Y + 25 – 50 25 – 50 10 – 25 25 – 50 100 50 37 80
Bso31 I
GGTCTCN↑
CCAGAG(N)5↓
 O + 25 – 50 75 – 100 100 75 – 100 25 – 50 40 55 80
Bsp13 I
T↑CCGGA
AGGCC↓T
 2K – 25 – 50 50 – 75 75 – 100 50 – 75 0 – 10 5 50 65
Bsp1720 I
GC↑TNAGC
CGANT↓CG
 G – 50 – 75 100 50 – 75 50 – 75 75 – 100 75 37 80
Bsp19 I
C↑CATGG
GGTAC↓C
 2W + 0 – 10 10 – 25 50 – 75 75 – 100 10 – 25 5 37 65
BspAC I
C↑CGC
GGC↓G
 O + 10 – 25 25 – 50 100 75 – 100 10 – 25 100 37 65
BspFN I
CG↑CG
GC↓GC
 Y – 50 – 75 75 – 100 75 – 100 50 – 75 100 100 37 65
BssEC I
C↑CNNGG
GGNNC↓C
 Y – 50 – 75 50 – 75 50 – 75 75 – 100 100 100 60 80
BssNA I
GTA↑TAC
CAT↓ATG
 W + 50 – 75 50 – 75 75 – 100 100 75 – 100 100 37 N
BssT1 I
C↑CWWGG
GGWWC↓C
 2K – 10 – 25 25 – 50 25 – 50 75 – 100 10 – 25 100 60 80
Bst2B I
C↑TCGTG
GAGCA↓C
 Y + 75 – 100 25 – 50 10 – 25 25 – 50 100 100 60 80
Bst2U I
CC↑WGG
GGW↓CC
 G + 75 – 100 100 50 – 75 50 – 75 10 – 25 50 60 N
Bst4C I
ACN↑GT
TG↓NCA
 Y – 75 – 100 75 – 100 10 – 25 25 – 50 100 50 65 N
Bst6 I
CTCTTCN↑
GAGAAG(N)4↓
 Y + 75 – 100 75 – 100 50 – 75 75 – 100 100 100 65 80
BstAC I
GR↑CGYC
CYGC↓RG
 W – 75 – 100 75 – 100 50 – 75 100 75 – 100 100 37 80
BstAF I
C↑TTAAG
GAATT↓C
 W + 10 – 25 25 – 50 75 – 100 100 25 – 50 100 55 80
BstAP I
GCANNNN↑NTGC
CGTN↓NNNNACG
 W – 25 – 50 25 – 50 75 – 100 100 25 – 50 100 60 80
BstAU I
T↑GTACA
ACATG↓T
 W – 10 – 25 50 – 75 25 – 50 100 25 – 50 100 37 80
BstBA I
YAC↑GTR
RTG↓CAY
 W + 25 – 50 25 – 50 75 – 100 100 25 – 50 50 65 80
BstC8 I
GCN↑NGC
CGN↓NCG
 Y – 10 – 25 25 – 50 50 – 75 75 – 100 100 100 55 80
BstDE I
C↑TNAG
GANT↓C
 G – 75 – 100 100 25 – 50 50 – 75 10 – 25 100 60 80
BstDS I
C↑CRYGG
GGYRC↓C
 Y – 0 – 10 75 – 100 50 – 75 25 – 50 100 100 65 80
BstEN I
CCTNN↑NNNAGG
GGANNN↓NNTCC
 Y – 50 – 75 50 – 75 25 – 50 25 – 50 100 100 65 80
BstF5 I
GGATGNN↑
CCTAC↓NN
 Y – 75 – 100 50 – 75 25 – 50 50 – 75 100 100 65 80
BstFN I
CG↑CG
GC↓GC
 Y – 75 – 100 50 – 75 25 – 50 25 – 50 100 75 60 80
BstH2 I
RGCGC↑Y
Y↓CGCGR
 Y + 50 – 75 50 – 75 0 – 10 10 – 25 100 100 65 80
BstHH I
GCG↑C
C↓GCG
 Y + 75 – 100 50 – 75 25 – 50 50 – 75 100 100 50 N
BstKT I
GAT↑C
C↓TAG
 W – 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
BstMA I
GTCTCN↑
CAGAG(N)5↓
 W + 25 – 50 50 – 75 50 – 75 100 75 – 100 100 55 65
BstMB I
↑GATC
CTAG↓
 O – 10 – 25 25 – 50 100 75 – 100 10 – 25 100 65 80
BstMC I
CGRY↑CG
GC↓YRGC
 B + 100 75 – 100 10 – 25 10 – 25 50 – 75 100 50 80
BstMW I
GCNNNNN↑NNGC
CGNN↓NNNNNCG
 Y – 10 – 25 25 – 50 25 – 50 50 – 75 100 40 55 80
BstNS I
RCATG↑Y
Y↓GTACR
 B + 100 50 – 75 10 – 25 10 – 25 75 – 100 50 37 65
BstPA I
GACNN↑NNGTC
CTGNN↓NNCAG
 Y – 50 – 75 25 – 50 50 – 75 50 – 75 100 100 65 N
BstSC I
↑CCNGG
GGNCC↓
 Y – 50 – 75 50 – 75 50 – 75 50 – 75 100 60 55 80
BstSF I
C↑TRYAG
GAYRT↓C
 O + 75 – 100 25 – 50 100 50 – 75 50 – 75 100 60 N
BstSL I
GKGCM↑C
C↓MCGKG
 G + 50 – 75 100 50 – 75 75 – 100 75 – 100 100 55 65
BstSN I
TAC↑GTA
ATG↓CAT
 B – 100 50 – 75 0 – 10 10 – 25 50 – 75 50 37 80
BstV1 I
GCAGC(N)8↑
CGTCG(N)12↓
 G – 75 – 100 100 75 – 100 75 – 100 75 – 100 100 55 80
BstV2 I
GAAGAC(N)2↑
CTTCTG(N)6↓
 Y + 75 – 100 75 – 100 25 – 50 25 – 50 100 70 55 65
BstX I
CCANNNNN↑NTGG
GGTN↓NNNNNACC
 O – 10 – 25 10 – 25 100 75 – 100 25 – 50 100 37 65
BstX2 I
R↑GATCY
YCTAG↓R
 G – 75 – 100 100 0 – 10 10 – 25 25 – 50 100 60 80
Bsu I
GTATCC(N)6↑
CATAGG(N)5↓
 Y – 75 – 100 50 – 75 10 – 25 25 – 50 100 10 37 65
BsuR I
GG↑CC
CC↓GG
 G – 75 – 100 100 25 – 50 50 – 75 50 – 75 100 37 80
Btr I
CAC↑GTC
GTG↓CAG
 O + 75 – 100 75 – 100 100 75 – 100 75 – 100 100 60 80
Cci I
T↑CATGA
AGTAC↓T
 W + 0 – 10 10 – 25 25 – 50 100 75 – 100 100 55 80
CciN I
GC↑GGCCGC
CGCCGG↓CG
 Y – 25 – 50 50 – 75 75 – 100 75 – 100 100 100 37 65
Dra I
TTT↑AAA
AAA↓TTT
 G + 75 – 100 100 25 – 50 75 – 100 75 – 100 100 37 65
Dra III
CACNNN↑GTG
GTG↓NNNCAC
 2K + 25 – 50 50 – 75 75 – 100 75 – 100 50 – 75 100 37 65
Dri I
GACNNN↑NNGTC
CTGNN↓NNNCAG
 Y – 75 – 100 75 – 100 10 – 25 10 – 25 100 40 37 65
DseD I
GACNNNN↑NNGTC
CTGNN↓NNNNCAG
 Y + 75 – 100 75 – 100 25 – 50 50 – 75 100 30 37 80
EcoICR I
GAG↑CTC
CTC↓GAG
 G + 75 – 100 100 0 – 10 0 – 10 75 – 100 5 37 65
EcoR I
G↑AATTC
CTTAA↓G
 * + 50 – 75 75 – 100 75 – 100 75 – 100 50 – 75 50 37 65
EcoR V
GAT↑ATC
CTA↓TAG
 W + 0 – 10 25 – 50 50 – 75 100 25 – 50 50 37 80
Ege I
GGC↑GCC
CCG↓CGG
 B + 100 75 – 100 10 – 25 50 – 75 75 – 100 100 37 65
Erh I
C↑CWWGG
GGWWC↓C
 2W + 10 – 25 25 – 50 50 – 75 75 – 100 10 – 25 100 37 65
Fae I
CATG↑
↓GTAC
 * + 25 – 50 50 – 75 10 – 25 10 – 25 75 – 100 100 37 65
Fai I
YA↑TR
RT↓AY
 B – 100 50 – 75 10 – 25 25 – 50 25 – 50 100 50 80
Fal I
↑(N)8AAGNNNNNCTT(N)13↑
↓(N)13TTCNNNNNGAA(N)8↓
 W+ SAM – 0 – 10 25 – 50 75 – 100 100 50 – 75 70 37 65
Fat I
↑CATG
GTAC↓
 G – 10 – 25 100 25 – 50 10 – 25 50 – 75 100 55 65
Fau I
CCCGC(N)4↑
GGGCG(N)6↓
 B – 100 25 – 50 0 – 10 0 – 10 50 – 75 50 55 65
FauND I
CA↑TATG
GTAT↓AC
 Y + 50 – 75 75 – 100 10 – 25 50 – 75 100 100 37 65
Fbl I
GT↑MKAC
CAKM↓TG
 Y – 50 – 75 75 – 100 0 – 10 50 – 75 100 100 55 80
Fok I
GGATG(N)9↑
CCTAC(N)13↓
 Y – 50 – 75 50 – 75 25 – 50 25 – 50 100 100 37 65
FriO I
GRGCY↑C
C↓YCGRG
 Y + 75 – 100 75 – 100 10 – 25 0 – 10 100 25 37 65
Fsp4H I
GC↑NGC
CGN↓CG
 Y – 50 – 75 75 – 100 10 – 25 25 – 50 100 100 37 65
Gsa I
CCCAG↑C
G↓GGTCG
 W + 10 – 25 25 – 50 75 – 100 100 75 – 100 100 70 N
Hae III
GG↑CC
CC↓GG
 G – 75 – 100 100 25 – 50 50 – 75 50 – 75 100 37 80
Hga I
GACGC(N)5↑
CTGCG(N)10↓
 B – 100 75 – 100 10 – 25 25 – 50 50 – 75 50 37 65
Hind II
GTY↑RAC
CAR↓YTG
 G + 75 – 100 100 25 – 50 25 – 50 75 – 100 50 37 65
Hind III
A↑AGCTT
TTCGA↓A
 W + 10 – 25 25 – 50 0 – 10 100 0 – 10 100 37 80
Hinf I
G↑ANTC
CTNA↓G
 O – 25 – 50 75 – 100 100 75 – 100 75 – 100 100 37 80
Hpa I
GTT↑AAC
CAA↓TTG
 Y – 0 – 10 50 – 75 10 – 25 25 – 50 100 25 37 65
Hpa II
C↑CGG
GGC↓C
 B – 100 50 – 75 10 – 25 25 – 50 50 – 75 50 37 80
HpySE526 I
A↑CGT
TGC↓A
 Y – 75 – 100 75 – 100 10 – 25 25 – 50 100 50 37 65
HspA I
G↑CGC
CGC↓G
 Y – 50 – 75 50 – 75 25 – 50 25 – 50 100 100 37 80
Kpn I
GGTAC↑C
C↓CATGG
 B + 100 25 – 50 25 – 50 25 – 50 75 – 100 50 37 80
Ksp22 I
T↑GATCA
ACTAG↓T
 2K + 50 – 75 75 – 100 50 – 75 50 – 75 25 – 50 100 37 65
Kzo9 I
↑GATC
CTAG↓
 G – 50 – 75 100 50 – 75 50 – 75 50 – 75 100 37 65
Lmn I
GCTCCN↑
CGAG↓GN
 B – 100 75 – 100 50 – 75 50 – 75 75 – 100 80 37 65
Mab I
A↑CCWGGT
TGGWCC↓A
 W + 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
Mbo II
GAAGA(N)8↑
CTTCT(N)7↓
 Y – 75 – 100 75 – 100 25 – 50 50 – 75 100 50 37 65
Mfe I
C↑AATTG
GTTAA↓C
 B + 100 75 – 100 10 – 25 25 – 50 75 – 100 25 37 N
Mhl I
GDGCH↑C
C↓HCGDG
 W – 10 – 25 25 – 50 75 – 100 100 10 – 25 100 37 80
Mlu I
A↑CGCGT
TGCGC↓A
 O – 0 – 10 10 – 25 100 25 – 50 10 – 25 50 37 65
Mly113 I
GG↑CGCC
CCGC↓GG
 B – 100 25 – 50 10 – 25 10 – 25 50 – 75 100 37 65
Mnl I
CCTC(N)7↑
GGAG(N)6↓
 G + 75 – 100 100 25 – 50 25 – 50 75 – 100 100 37 65
Mox20 I
TGG↑CCA
ACC↓GGT
 O – 10 – 25 25 – 50 100 75 – 100 25 – 50 75 37 N
MroN I
G↑CCGGC
CGGCC↓G
 B – 100 50 – 75 10 – 25 0 – 10 10 – 25 5 37 80
MroX I
GAANN↑NNTTC
CTTNN↓NNAAG
 W – 50 – 75 50 – 75 50 – 75 100 25 – 50 100 37 65
Msp I
C↑CGG
GGC↓C
 B – 100 75 – 100 50 – 75 75 – 100 75 – 100 100 37 65
MspA1 I
CMG↑CKG
GKC↓GMC
 Y + 10 – 25 75 – 100 10 – 25 25 – 50 100 100 37 65
MspR9 I
CC↑NGG
GGN↓CC
 O – 50 – 75 50 – 75 100 50 – 75 50 – 75 100 37 80
Nru I
TCG↑CGA
AGC↓GCT
 W – 0 – 10 10 – 25 75 – 100 100 10 – 25 100 37 80
PalA I
GG↑CGCGCC
CCGCGC↓GG
 Y – 25 – 50 10 – 25 75 – 100 100 100 40 37 65
Pce I
AGG↑CCT
TCC↓GGA
 Y – 75 – 100 75 – 100 50 – 75 25 – 50 100 100 50 80
Pci I
A↑CATGT
TGTAC↓A
 O – 50 – 75 75 – 100 100 75 – 100 50 – 75 50 37 65
PciS I
GCTCTTCN↑
CGAGAAG(N)4↓
 B – 100 50 – 75 0 – 10 0 – 10 75 – 100 50 37 65
Pct I
GAATGCN↑
CTTAC↓GN
 O – 25 – 50 50 – 75 100 75 – 100 10 – 25 50 37 65
Ple19 I
CGAT↑CG
GC↓TAGC
 Y – 75 – 100 75 – 100 25 – 50 25 – 50 100 100 37 65
Pps I
GAGTC(N)4↑
CTCAG(N)5↓
 Y + 50 – 75 10 – 25 0 – 10 25 – 50 100 50 37 65
Psi I
TTA↑TAA
AAT↓ATT
 B – 100 25 – 50 10 – 25 25 – 50 75 – 100 40 37 65
Psp124B I
GAGCT↑C
C↓TCGAG
 G – 75 – 100 100 10 – 25 0 – 10 75 – 100 30 37 80
Psp6 I
↑CCWGG
GGWCC↓
 B – 100 50 – 75 10 – 25 25 – 50 75 – 100 10 55 80
PspC I
CAC↑GTG
GTG↓CAC
 B + 100 50 – 75 0 – 10 0 – 10 50 – 75 5 37 65
PspE I
G↑GTNACC
CCANTG↓G
 B – 100 50 – 75 25 – 50 50 – 75 50 – 75 100 37 65
PspL I
C↑GTACG
GCATG↓C
 Y + 75 – 100 75 – 100 25 – 50 10 – 25 100 100 37 65
PspN4 I
GGN↑NCC
CCN↓NGG
 Y – 10 – 25 10 – 25 10 – 25 25 – 50 100 50 37 65
PspOM I
G↑GGCCC
CCCGG↓G
 Y – 75 – 100 10 – 25 0 – 10 0 – 10 100 25 37 65
PspPP I
RG↑GWCCY
YCCWG↓GR
 Y + 10 – 25 10 – 25 0 – 10 0 – 10 100 100 37 65
PspX I
VC↑TCGAGB
BGAGCT↓CV
 Y + 50 – 75 50 – 75 25 – 50 75 – 100 100 25 37 80
Psr I
↑(N)7GAACNNNNNNTAC(N)12↑
↓(N)12CTTGNNNNNNATG(N)7↓
 Y + 10 – 25 10 – 25 0 – 10 0 – 10 100 30 30 65
Pst I
CTGCA↑G
G↓ACGTC
 O + 10 – 25 25 – 50 100 25 – 50 25 – 50 50 37 80
PstN I
CAGNNN↑CTG
GTC↓NNNGAC
 Y – 25 – 50 50 – 75 10 – 25 25 – 50 100 100 37 65
Pvu II
CAG↑CTG
GTC↓GAC
 G + 25 – 50 100 25 – 50 25 – 50 25 – 50 100 37 80
Rga I
GCGAT↑CGC
CGC↓TAGCG
 Y – 75 – 100 50 – 75 10 – 25 25 – 50 100 100 55 80
Rig I
GGCCGG↑CC
CC↓GGCCGG
 * + 75 – 100 50 – 75 0 – 10 10 – 25 50 – 75 10 37 65
Rsa I
GT↑AC
CA↓TG
 B – 100 50 – 75 0 – 10 50 – 75 75 – 100 50 37 N
RsaN I
G↑TAC
CAT↓G
 B – 100 75 – 100 50 – 75 50 – 75 75 – 100 100 37 80
Rsr2 I
CG↑GWCCG
GCCWG↓GC
 Y + 50 – 75 75 – 100 0 – 10 10 – 25 100 25 37 65
Sal I
G↑TCGAC
CAGCT↓G
 O – 0 – 10 10 – 25 100 25 – 50 0 – 10 5 37 65
Sbf I
CCTGCA↑GG
GG↓ACGTCC
 Y – 75 – 100 50 – 75 0 – 10 0 – 10 100 50 37 80
Set I
ASST↑
↓TSSA
 Y – 25 – 50 25 – 50 75 – 100 75 – 100 100 100 55 80
SfaN I
GCATC(N)5↑
CGTAG(N)9↓
 O – 10 – 25 25 – 50 100 75 – 100 0 – 10 25 37 80
Sfi I
GGCCNNNN↑NGGCC
CCGGN↓NNNNCCGG
 G + 75 – 100 100 25 – 50 25 – 50 25 – 50 75 50 65
Sfr274 I
C↑TCGAG
GAGCT↓C
 B – 100 75 – 100 50 – 75 50 – 75 75 – 100 100 50 65
Sfr303 I
CCGC↑GG
GG↓CGCC
 B – 100 50 – 75 10 – 25 10 – 25 75 – 100 100 37 65
Sma I
CCC↑GGG
GGG↓CCC
 Y – 0 – 10 0 – 10 0 – 10 0 – 10 100 50 25 65
Smi I
ATTT↑AAAT
TAAA↓TTTA
 O + 25 – 50 25 – 50 100 75 – 100 25 – 50 10 37 65
SmiM I
CAYNN↑NNRTG
GTRNN↓NNYAC
 W – 10 – 25 10 – 25 75 – 100 100 10 – 25 60 37 65
Sph I
GCATG↑C
C↓GTACG
 G + 25 – 50 100 75 – 100 75 – 100 50 – 75 100 37 65
Sse9 I
↑AATT
TTAA↓
 B + 100 75 – 100 50 – 75 50 – 75 75 – 100 75 55 65
Ssp I
AAT↑ATT
TTA↓TAA
 K + 75 – 100 50 – 75 25 – 50 50 – 75 75 – 100 100 37 65
Taq I
T↑CGA
AGC↓T
 Y + 50 – 75 75 – 100 75 – 100 50 – 75 100 100 65 80
Tru9 I
T↑TAA
AAT↓T
 W – 75 – 100 25 – 50 25 – 50 100 50 – 75 100 65 80
TseF I
↑GTSAC
CASTG↓
 B – 100 50 – 75 0 – 10 25 – 50 50 – 75 50 65 N
Tth111 I
GACN↑NNGTC
CTGNN↓NCAG
 Y – 75 – 100 50 – 75 10 – 25 10 – 25 100 100 65 80
Vne I
G↑TGCAC
CACGT↓G
 O – 10 – 25 25 – 50 100 25 – 50 25 – 50 100 37 65
Vsp I
AT↑TAAT
TAAT↓TA
 W – 0 – 10 10 – 25 50 – 75 100 25 – 50 50 37 65
Xba I
T↑CTAGA
AGATC↓T
 O + 75 – 100 75 – 100 100 50 – 75 75 – 100 25 37 65
Xma I
C↑CCGGG
GGGCC↓C
 Y – 75 – 100 50 – 75 0 – 10 0 – 10 100 50 37 65
Zra I
GAC↑GTC
CTG↓CAG
 B – 100 50 – 75 25 – 50 25 – 50 75 – 100 100 37 80
Zrm I
AGT↑ACT
TCA↓TGA
 Y + 50 – 75 25 – 50 0 – 10 0 – 10 100 50 37 65
Zsp2 I
ATGCA↑T
T↓ACGTA
 B + 100 50 – 75 25 – 50 25 – 50 25 – 50 50 37 65

Изошизомеры

Алфавитный указатель прототипов и коммерчески доступных изошизомеров эндонуклеаз рестрикции

Прототип Фермент SibEnzyme Прототип Фермент SibEnzyme Прототип Фермент SibEnzyme
AasI DseDI BsrGI BstAUI MboI BstKTI^
BstMBI
Kzo9I
AatI PceI BsrI Bse1I MboII MboII
AatII AatII
ZraI^		 BsrSI Bse1I McrI BstMCI
AbsI AbsI BssAI Bse118I MfeI MfeI
Acc16I Acc16I BssECI BssECI MflI BstX2I
Acc36I Acc36I BssHI Sfr274I MhlI MhlI
Acc65I Acc65I
KpnI^		 BssHII BsePI MlsI Msp20I
AccB1I AccB1I BssKI BstSCI
MspR9I^		 MluI MluI
AccB7I AccB7I BssNAI BssNAI MluNI Msp20I
AccBSI AccBSI BssSI Bst2BI Mly113I EgeI^
Mly113I
AccI FblI BssT1I BssT1I
ErhI		 MlyI PpsI^
AccII BstFNI Bst1107I BssNAI MnlI MnlI
AccIII Bsp13I Bst2BI Bst2BI Mph1103I Zsp2I
AciI BspACI Bst2UI AjnI^
Bst2UI
Psp6I^		 MroI Bsp13I
AclI AclI Bst4CI Bst4CI MroNI MroNI
AclWI AclWI Bst6I Bst6I MroXI MroXI
AcoI AcoI Bst98I BstAFI MscI Msp20I
AcsI AcsI BstACI BstACI MseI Tru9I
AcuI AcuI BstAFI BstAFI MslI SmiMI
AcvI PspCI BstAPI BstAPI Msp20I Msp20I
AcyI BstACI BstAUI BstAUI MspA1I MspA1I
AdeI DraIII BstBAI BstBAI MspCI BstAFI
AfaI RsaI BstBI Bpu14I MspI HpaII
MspI
AfeI AfeI BstC8I BstC8I MspR9I BstSCI^
MspR9I
AflII BstAFI BstDEI BstDEI MstI Acc16I
AgeI AsiGI BstDSI BstDSI MunI MfeI
AhaIII DraI BstEII PspEI Mva1269I PctI
AhdI DriI BstENI BstENI MvaI AjnI^
Bst2UI
Psp6I^
AhlI AhlI BstF5I BstF5I
FokI^		 MvnI BstFNI
AjnI AjnI
Bst2UI^
Psp6I		 BstFNI BstFNI MvrI Ple19I
AluI AluI BstH2I BstH2I MwoI BstMWI
Alw21I Bbv12I BstHHI AspLEI
BstHHI
HspAI^		 NaeI MroNI^
Alw26I BstMAI BstKTI BstKTI
BstMBI^
Kzo9I^		 NarI EgeI^
Mly113I
Alw44I VneI BstMAI BstMAI NciI AsuC2I
AlwI AclWI BstMBI BstKTI^
BstMBI
Kzo9I		 NcoI Bsp19I
AlwNI PstNI BstMCI BstMCI NdeI FauNDI
Ama87I Ama87I BstMWI BstMWI NdeII BstKTI^
BstMBI
Kzo9I
Aor13HI Bsp13I BstNI AjnI^
Bst2UI
Psp6I^		 NgoMIV MroNI
Aor51HI AfeI BstNSI BstNSI NheI AsuNHI
BmtI^
AoxI AoxI BstOI AjnI^
Bst2UI
Psp6I^		 NlaIII FatI^
FaeI
ApaBI BstAPI^ BstPAI BstPAI NlaIV PspN4I
ApaI ApaI
PspOMI^		 BstPI PspEI NotI CciNI
ApaLI VneI BstSCI BstSCI
MspR9I^		 NruI NruI
ApoI AcsI BstSFI BstSFI NsbI Acc16I
AscI PalAI BstSNI BstSNI NsiI Zsp2I
AseI VspI BstUI BstFNI NspBII MspA1I
AsiGI AsiGI BstV1I BstV1I NspI BstNSI
AsiSI RgaI
AsiSI		 BstV2I BstV2I NspIII Ama87I
AsoI PalAI BstX2I BstX2I NspV Bpu14I
Asp700I MroXI BstXI BstXI PaeI SphI
Asp718I Acc65I
KpnI^		 BstYI BstX2I PaeR7I Sfr274I
AspA2I AspA2I BstZ17I BssNAI PalAI PalAI
AspEI DriI BstZI BseX3I PauI BsePI
AspI Tth111I Bsu15I Bsa29I PceI PceI
AspLEI AspLEI
BstHHI
HspAI^		 Bsu36I Bse21I PciI PciI
AspS9I AspS9I BsuRI BsuRI
HaeIII		 PciSI PciSI
AssI ZrmI BsuTUI Bsa29I PctI PctI
AsuC2I AsuC2I BtgI BstDSI PdiI MroNI^
AsuHPI AsuHPI BtrI BtrI PdmI MroXI
AsuI AspS9I BveI Acc36I Pfl23II PspLI
AsuII Bpu14I Cac8I BstC8I PflBI AccB7I
AsuNHI AsuNHI
BmtI^		 CauII AsuC2I PflFI Tth111I
AvaI Ama87I CciNI CciNI PflMI AccB7I
AvaII Bme18I CelII Bsp1720I PhoI BsuRI
HaeIII
AvaIII Zsp2I^ CfoI AspLEI
BstHHI
HspAI^		 PinAI AsiGI
AviII Acc16I Cfr10I Bse118I Ple19I Ple19I
AvrII AspA2I Cfr13I AspS9I PleI PpsI
AxyI Bse21I Cfr42I Sfr303I PmaCI PspCI
BalI Msp20I Cfr9I SmaI^
XmaI		 PmlI PspCI
BamHI BamHI CfrI AcoI PpsI PpsI
BanI AccB1I ClaI Bsa29I PpuMI PspPPI
BanII FriOI CpoI Rsr2I PpuXI PspPPI
BanIII Bsa29I CseI HgaI PscI PciI
BarI BarI Csp45I Bpu14I PshAI BstPAI
BauI Bst2BI Csp6I RsaI^ PshBI VspI
BbeI EgeI^
Mly113I^		 CspAI AsiGI PsiI PsiI
BbrPI PspCI CspI Rsr2I Psp124BI EcoICRI^
Psp124BI
BbsI BstV2I CviAII FatI^
FaeI^		 Psp1406I AclI
BbuI SphI DdeI BstDEI Psp5II PspPPI
Bbv12I Bbv12I DpnI BstKTI^
BstMBI^
Kzo9I^		 Psp6I AjnI
Bst2UI^
Psp6I
BbvI BstV1I DpnII BstKTI^
BstMBI
Kzo9I		 PspCI PspCI
BbvII BstV2I DraI DraI PspEI PspEI
BciVI BsuI DraIII DraIII PspGI AjnI
Bst2UI^
Psp6I
BclI Ksp22I DrdI DseDI PspLI PspLI
BcnI AsuC2I DriI DriI PspN4I PspN4I
BcuI AhlI DsaI BstDSI PspOMI ApaI^
PspOMI
BfiI BmuI DseDI DseDI PspPI AspS9I
BfmI BstSFI EaeI AcoI PspPPI PspPPI
BfrBI Zsp2I^ EagI BseX3I PspXI PspXI
BfrI BstAFI Eam1104I Bst6I PsrI PsrI
BfuAI Acc36I Eam1105I DriI PstI PstI
BfuCI BstKTI^
BstMBI
Kzo9I		 EarI Bst6I PsuI BstX2I
BglI BglI Ecl136II EcoICRI
Psp124BI^		 PsyI Tth111I
BglII BglII EclHKI DriI PvuI Ple19I
BinI AclWI EclXI BseX3I PvuII PvuII
BisI BisI
BlsI^		 Eco105I BstSNI RgaI RgaI
AsiSI
BlfI Bsp13I Eco130I BssT1I
ErhI		 RigI RigI
BlnI AspA2I Eco147I PceI RsaI RsaI
BlpI Bsp1720I Eco24I FriOI Rsr2I Rsr2I
BlsI BisI^
BlsI		 Eco31I Bso31I RsrII Rsr2I
Bme1390I BstSCI^
MspR9I		 Eco32I EcoRV SacI EcoICRI^
Psp124BI
Bme18I Bme18I Eco47I Bme18I SacII Sfr303I
BmgBI BtrI Eco47III AfeI SalI SalI
BmrI BmuI Eco52I BseX3I SapI PciSI
BmtI AsuNHI^
BmtI		 Eco57I AcuI SatI Fsp4HI
BmuI BmuI Eco72I PspCI Sau3AI BstKTI^
BstMBI
Kzo9I
BoxI BstPAI Eco81I Bse21I Sau96I AspS9I
BpcI BstSFI Eco88I Ama87I SauI Bse21I
BpiI BstV2I Eco91I PspEI SbfI SbfI
BpmI BpmI EcoICRI EcoICRI
Psp124BI^		 ScaI ZrmI
BptI AjnI^
Bst2UI
Psp6I^		 EcoNI BstENI SchI PpsI^
Bpu10I Bpu10I EcoO65I PspEI ScrFI BstSCI^
MspR9I
Bpu1102I Bsp1720I EcoRI EcoRI SdaI SbfI
Bpu14I Bpu14I EcoRII AjnI
Bst2UI^
Psp6I		 SduI MhlI
BpuAI BstV2I EcoRV EcoRV SecI BssECI
Bsa29I Bsa29I EcoT14I BssT1I
ErhI		 SetI SetI
BsaAI BstBAI EcoT22I Zsp2I SexAI MabI
BsaBI Bse8I EcoT38I FriOI SfaNI SfaNI
BsaHI BstACI EgeI EgeI
Mly113I^		 SfcI BstSFI
BsaI Bso31I EheI EgeI
Mly113I^		 SfeI BstSFI
BsaJI BssECI ErhI BssT1I
ErhI		 SfiI SfiI
BsaMI PctI EspI Bsp1720I SfoI EgeI
Mly113I^
Bsc4I Bsc4I FaeI FatI^
FaeI		 Sfr274I Sfr274I
Bse118I Bse118I FalI FalI Sfr303I Sfr303I
Bse1I Bse1I FaqI BslFI SfuI Bpu14I
Bse21I Bse21I FatI FatI
FaeI^		 SgfI RgaI
AsiSI
Bse3DI Bse3DI FauI FauI SgrBI Sfr303I
Bse8I Bse8I FauNDI FauNDI SgsI PalAI
BseAI Bsp13I FbaI Ksp22I SinI Bme18I
BseBI AjnI^
Bst2UI
Psp6I^		 FblI FblI SlaI Sfr274I
BseCI Bsa29I FinI BslFI^ SmaI SmaI
XmaI^
BseDI BssECI Fnu4HI Fsp4HI SmiI SmiI
BseGI BstF5I
FokI^		 FnuDII BstFNI SmiMI SmiMI
BseJI Bse8I FokI BstF5I^
FokI		 SmuI FauI
BseLI Bsc4I FriOI FriOI SnaBI BstSNI
BseMI Bse3DI FseI RigI SnaI BssNAI^
BseNI Bse1I Fsp4HI Fsp4HI SpaHI SphI
BsePI BsePI FspI Acc16I SpeI AhlI
BseX3I BseX3I FunI AfeI SphI SphI
BseXI BstV1I FunII EcoRI SplI PspLI
Bsh1236I BstFNI GlaI GlaI Sse8387I SbfI
Bsh1285I BstMCI GluI GluI Sse9I Sse9I
BshFI BsuRI
HaeIII		 GsuI BpmI SseBI PceI
BshNI AccB1I HaeII BstH2I SsiI BspACI
BshTI AsiGI HaeIII BsuRI
HaeIII		 SspBI BstAUI
BsiEI BstMCI HapII HpaII
MspI		 SspI SspI
BsiHKAI Bbv12I HgaI HgaI SstI EcoICRI^
Psp124BI
BsiHKCI Ama87I HgiAI Bbv12I SstII Sfr303I
BsiI Bst2BI HgiCI AccB1I StrI Sfr274I
BsiSI HpaII
MspI		 HgiJII FriOI StuI PceI
BsiWI PspLI HhaI AspLEI
BstHHI
HspAI^		 StyD4I BstSCI
MspR9I^
BsiYI Bsc4I Hin1I BstACI StyI BssT1I
ErhI
BslFI BslFI Hin1II FatI^
FaeI		 SunI PspLI
BslI Bsc4I Hin6I AspLEI^
BstHHI^
HspAI		 SwaI SmiI
BsmAI BstMAI HincII HindII TaaI Bst4CI
BsmFI BslFI HindII HindII TaqI TaqI
BsmI PctI HindIII HindIII TasI Sse9I
Bso31I Bso31I HinfI HinfI TelI Tth111I
BsoBI Ama87I HinP1I AspLEI^
BstHHI^
HspAI		 TliI Sfr274I
Bsp119I Bpu14I HpaI HpaI Tru1I Tru9I
Bsp120I ApaI^
PspOMI		 HpaII HpaII
MspI		 Tru9I Tru9I
Bsp1286I MhlI HphI AsuHPI Tsp45I TseFI
Bsp13I Bsp13I HpyCH4III Bst4CI Tsp4CI Bst4CI
Bsp1407I BstAUI HpyF10VI BstMWI Tsp509I Sse9I
Bsp143I BstKTI^
BstMBI
Kzo9I		 HpyF3I BstDEI TspEI Sse9I
Bsp143II BstH2I Hsp92I BstACI Tth111I Tth111I
Bsp1720I Bsp1720I Hsp92II FatI^
FaeI		 Van91I AccB7I
Bsp19I Bsp19I HspAI AspLEI^
BstHHI^
HspAI		 VneI VneI
Bsp68I NruI ItaI Fsp4HI VpaK11BI Bme18I
BspACI BspACI KasI EgeI^
Mly113I^		 VspI VspI
BspANI BsuRI
HaeIII		 Kpn2I Bsp13I XagI BstENI
BspDI Bsa29I KpnI Acc65I^
KpnI		 XapI AcsI
BspEI Bsp13I KroI KroI XbaI XbaI
BspLI PspN4I Ksp22I Ksp22I XceI BstNSI
BspLU11I PciI Ksp632I Bst6I XhoI Sfr274I
BspMAI PstI KspAI HpaI XhoII BstX2I
BspMI Acc36I KspI Sfr303I XmaCI SmaI^
XmaI
BspMII Bsp13I Kzo9I BstKTI^
BstMBI
Kzo9I		 XmaI SmaI^
XmaI
BspPI AclWI LguI PciSI XmaIII BseX3I
BspT104I Bpu14I LweI SfaNI XmaJI AspA2I
BspT107I AccB1I MabI MabI XmiI FblI
BspTI BstAFI MaeI SspMI XmnI MroXI
BspTNI Bso31I MaeII HpySE526I ZhoI Bsa29I
BspXI Bsa29I MalI MalI ZraI AatII^
ZraI
BsrBI AccBSI MamI Bse8I ZrmI ZrmI
BsrDI Bse3DI MbiI AccBSI Zsp2I Zsp2I
BsrFI Bse118I

Чувствительность к метилированию

Блокирование активности рестриктаз при полном или частичном перекрывании их сайтов узнавания метилированием

 

Рестриктаза

Позиция гидролиза

Прототип

Блокирование
рестриктазы при полном или частичном перекрывании её сайта узнавания
метилированием

сопряжённой ДНК
метилазой

dam
Gm6ATC

dcm
Cm5CWGG

M.SssI
m5CG

AatII

GACGT↑C
C↓TGCAG

AatII

G(m6A)CGTC

AbsI

CC↑TCGAGG
GGAGCT↓CC

AbsI

Acc16I

TGC↑GCA
ACG↓CGT

MstI

Acc36I

ACCTGC(N)4↑
TGGACG(N)8↓

BspMI

Acc65I

G↑GTACC
CCATG↓G

KpnI

+

AccB1I

G↑GYRCC
CCRYG↓G

HgiCI

AccB7I

CCANNNN↑NTGG
GGTN↓NNNNACC

PflMI

+

AccBSI

GAG↑CGG
CTC↓GCC

BsrBI

AclI

AA↑CGTT
TTGC↓AA

AclI

+

AclWI

GGATC(N)4↑
CCTAG(N)5↓

BinI

+

AcoI

Y↑GGCCR
RCCGG↓Y

CfrI

+

AcsI

R↑AATTY
YTTAA↓R

ApoI

AcuI

CTGAAG(N)16↑
GACTTC(N)14↓

Eco57I

AfeI

AGC↑GCT
TCG↓CGA

Eco47III

AgsI

TTS↑AA
AA↓STT

AgsI

TTSA(m6A)

AhlI

A↑CTAGT
TGATC↓A

SpeI

AjnI

↑CCWGG
GGWCC↓

EcoRII

(m5C)CWGG

–

AluI

AG↑CT
TC↓GA

AluI

AG(m5C)T

AluBI

AG↑CT
TC↓GA

AluI

(m6A)GCT

Ama87I

C↑YCGRG
GRGCY↓C

AvaI

AoxI

↑RG(5mC)Y
Y(5mC)GR↓

AoxI

ApaI

GGGCC↑C
C↓CCGGG

ApaI

+

ArsI

↑(N)8GACNNNNNNTTYG(N)11↑
↓(N)13CTGNNNNNNAARC(N)6↓

ArsI

AsiGI

A↑CCGGT
TGGCC↓A

AgeI

AsiSI

GCGAT↑CGC
CGC↓TAGCG

SgfI

–

+

AspA2I

C↑CTAGG
GGATC↓C

AvrII

AspLEI

GCG↑C
C↓GCG

HhaI

+

AspS9I

G↑GNCC
CCNG↓G

Sau96I

+

AsuC2I

CC↑SGG
GGS↓CC

CauII

AsuHPI

GGTGA(N)8↑
CCACT(N)7↓

HphI

+

AsuNHI

G↑CTAGC
CGATC↓G

NheI

BamHI

G↑GATCC
CCTAG↓G

BamHI

GGAT(m4C)C

–

BarI

↑(N)7GAAGNNNNNNTAC(N)12↑
↓(N)12CTTCNNNNNNATG(N)7↓

BarI

Bbv12I

GWGCW↑C
C↓WCGWG

HgiAI

BglI

GCCNNNN↑NGGC
CGGN↓NNNNCCG

BglI

–

BglII

A↑GATCT
TCTAG↓A

BglII

–

BlsI

Последовательность
ДНК,
содержащая не менее двух 5mC:
RYN↑RY
RYN↓RY

BlsI

Bme18I

G↑GWCC
CCWG↓G

AvaII

+

BmtI

GCTAG↑C
C↓GATCG

NheI

BmuI

ACTGGG(N)5↑
TGACCC(N)4↓

BfiI

BpmI

CTGGAG(N)16↑
GACCTC(N)14↓

GsuI

Bpu10I

CC↑TNAGC
GGANT↓CG

Bpu10I

Bpu14I

TT↑CGAA
AAGC↓TT

AsuII

Bsa29I

AT↑CGAT
TAGC↓TA

ClaI

+

Bsc4I

CCNNNNN↑NNGG
GGNN↓NNNNNCC

BsiYI

Bse1I

ACTGGN↑
TGAC↓CN

BsrI

Bse118I

R↑CCGGY
YGGCC↓R

Cfr10I

Bse21I

CC↑TNAGG
GGANT↓CC

SauI

Bse3DI

GCAATGNN↑
CGTTAC↓NN

BsrDI

Bse8I

GATNN↑NNATC
CTANN↓NNTAG

BsaBI

BsePI

G↑CGCGC
CGCGC↓G

BsePI

BseX3I

C↑GGCCG
GCCGG↓C

XmaIII

BslFI

GGGAC(N)10↑
CCCTG(N)14↓

FinI

Bso31I

GGTCTCN↑
CCAGAG(N)5↓

Eco31I

Bsp13I

T↑CCGGA
AGGCC↓T

BspMII

+

Bsp1720I

GC↑TNAGC
CGANT↓CG

EspI

Bsp19I

C↑CATGG
GGTAC↓C

NcoI

(m4C)CATGG
(m5C)CATGG/GGTAC(m5C)

BspACI

C↑CGC
GGC↓G

AciI

+

BspFNI

CG↑CG
GC↓GC

FnuDII

+

BssECI

C↑CNNGG
GGNNC↓C

SecI

(m4C)CNNGG

BssNAI

GTA↑TAC
CAT↓ATG

SnaI

BssT1I

C↑CWWGG
GGWWC↓C

StyI

Bst2BI

C↑TCGTG
GAGCA↓C

BsiI

Bst2UI

CC↑WGG
GGW↓CC

BstNI

–

Bst4CI

ACN↑GT
TG↓NCA

Tsp4CI

Bst6I

CTCTTCN↑
GAGAAG(N)4↓

Ksp632I

BstACI

GR↑CGYC
CYGC↓RG

AcyI

BstAFI

C↑TTAAG
GAATT↓C

AflII

BstAPI

GCANNNN↑NTGC
CGTN↓NNNNACG

(ApaBI)

BstAUI

T↑GTACA
ACATG↓T

Bsp1407I

BstBAI

YAC↑GTR
RTG↓CAY

BsaAI

+

BstC8I

GCN↑NGC
CGN↓NCG

Cac8I

G(m5C)NNGC

BstDEI

C↑TNAG
GANT↓C

DdeI

BstDSI

C↑CRYGG
GGYRC↓C

DsaI

BstENI

CCTNN↑NNNAGG
GGANNN↓NNTCC

EcoNI

–

BstF5I

GGATGNN↑
CCTAC↓NN

(FokI)

BstFNI

CG↑CG
GC↓GC

FnuDII

+

BstH2I

RGCGC↑Y
Y↓CGCGR

HaeII

BstHHI

GCG↑C
C↓GCG

HhaI

+

BstKTI

GAT↑C
C↓TAG

MboI

+

BstMAI

GTCTCN↑
CAGAG(N)5↓

BsmAI

BstMBI

↑GATC
CTAG↓

MboI

+

BstMCI

CGRY↑CG
GC↓YRGC

McrI

BstMWI

GCNNNNN↑NNGC
CGNN↓NNNNNCG

MwoI

G(m5C)NNNNNNNGC

BstNSI

RCATG↑Y
Y↓GTACR

NspI

BstPAI

GACNN↑NNGTC
CTGNN↓NNCAG

PshAI

BstSCI

↑CCNGG
GGNCC↓

ScrFI

+

BstSFI

C↑TRYAG
GAYRT↓C

SfeI

BstSLI

GKGCM↑C
C↓MCGKG

BseSI

GKG(m5C)MC

–

BstSNI

TAC↑GTA
ATG↓CAT

SnaBI

BstV1I

GCAGC(N)8↑
CGTCG(N)12↓

BbvI

BstV2I

GAAGAC(N)2↑
CTTCTG(N)6↓

BbvII

BstXI

CCANNNNN↑NTGG
GGTN↓NNNNNACC

BstXI

BstX2I

R↑GATCY
YCTAG↓R

XhoII

–

BstX2I

R↑GATCY
YCTAG↓R

XhoII

BsuI

GTATCC(N)6↑
CATAGG(N)5↓

BciVI

BsuRI

GG↑CC
CC↓GG

HaeIII

BtrI

CAC↑GTC
GTG↓CAG

BtrI

CciI

T↑CATGA
AGTAC↓T

BspHI

CciNI

GC↑GGCCGC
CGCCGG↓CG

NotI

+

DraI

TTT↑AAA
AAA↓TTT

AhaIII

DraIII

CACNNN↑GTG
GTG↓NNNCAC

DraIII

DriI

GACNNN↑NNGTC
CTGNN↓NNNCAG

Eam1105I

DseDI

GACNNNN↑NNGTC
CTGNN↓NNNNCAG

DrdI

EcoICRI

GAG↑CTC
CTC↓GAG

SacI

EcoRI

G↑AATTC
CTTAA↓G

EcoRI

GA(m6A)TTC

EcoRV

GAT↑ATC
CTA↓TAG

EcoRV

EgeI

GGC↑GCC
CCG↓CGG

NarI

ErhI

C↑CWWGG
GGWWC↓C

StyI

FaeI

CATG↑
↓GTAC

NlaIII

C(m6A)TG

FaiI

YA↑TR
RT↓AY

FaiI

FalI

↑(N)8AAGNNNNNCTT(N)13↑
↓(N)13TTCNNNNNGAA(N)8↓

FalI

FatI

↑CATG
GTAC↓

NlaIII

(m5C)ATG

FauI

CCCGC(N)4↑
GGGCG(N)6↓

FauI

+

FauNDI

CA↑TATG
GTAT↓AC

NdeI

FblI

GT↑MKAC
CAKM↓TG

AccI

FokI

GGATG(N)9↑
CCTAC(N)13↓

FokI

FriOI

GRGCY↑C
C↓YCGRG

BanII

Fsp4HI

GC↑NGC
CGN↓CG

Fnu4HI

G(m5C)NGC

GlaI

R(5mC)↑GY
YG↓(5mC)R

GlaI

GluI

G(5mC)↑NG(5mC)
(5mC)GN↓(5mC)G

GluI

GsaI

CCCAG↑C
G↓GGTCG

BseYI

HaeIII

GG↑CC
CC↓GG

HaeIII

GG(m5C)C

HgaI

GACGC(N)5↑
CTGCG(N)10↓

HgaI

+

HindII

GTY↑RAC
CAR↓YTG

HindII

HindIII

A↑AGCTT
TTCGA↓A

HindIII

HinfI

G↑ANTC
CTNA↓G

HinfI

HpaI

GTT↑AAC
CAA↓TTG

HpaI

+

HpaII

C↑CGG
GGC↓C

HpaII

C(m5C)GG, а так же
MspI-метилированием:
(m5C)CGG

+

HpySE526I

A↑CGT
TGC↓A

MaeII

HspAI

G↑CGC
CGC↓G

HhaI

+

KpnI

GGTAC↑C
C↓CATGG

KpnI

–

KroI

G↑C(5mC)GGC
CGG(5mC)C↓G

KroI

KroNI

GCC↑GGC
CGG↓CCG

NaeI

+

Ksp22I

T↑GATCA
ACTAG↓T

BclI

+

Kzo9I

↑GATC
CTAG↓

MboI

–

+
GAT(m5C)G

LmnI

GCTCCN↑
CGAG↓GN

LmnI

MabI

A↑CCWGGT
TGGWCC↓A

SexAI

–

MalI

G(mA)↑TC
CT↓(mA)G

DpnI

MboII

GAAGA(N)8↑
CTTCT(N)7↓

MboII

+

MfeI

C↑AATTG
GTTAA↓C

MfeI

MhlI

GDGCH↑C
C↓HCGDG

SduI

MluI

A↑CGCGT
TGCGC↓A

MluI

Mly113I

GG↑CGCC
CCGC↓GG

NarI

MnlI

CCTC(N)7↑
GGAG(N)6↓

MnlI

+
CCT(m5C)G

Mox20I

TGG↑CCA
ACC↓GGT

BalI

+

MroNI

G↑CCGGC
CGGCC↓G

(NaeI)

MroXI

GAANN↑NNTTC
CTTNN↓NNAAG

XmnI

MspI

C↑CGG
GGC↓C

HpaII

(m5C)CGG

–

MspA1I

CMG↑CKG
GKC↓GMC

NspBII

MspR9I

CC↑NGG
GGN↓CC

ScrFI

+

MteI

G(5mC)G(5mC)↑NG(5mC)G(5mC)
(5mC)G(5mC)GN↓(5mC)G(5mC)G

Mte

NruI

TCG↑CGA
AGC↓GCT

NruI

+

PalAI

GG↑CGCGCC
CCGCGC↓GG

AscI

+

PceI

AGG↑CCT
TCC↓GGA

StuI

PciI

A↑CATGT
TGTAC↓A

BspLU11I

(m6A)CATGT

PciSI

GCTCTTCN↑
CGAGAAG(N)4↓

SapI

PcsI

(5mC)GNNNNN↑NN(5mC)G
G(5mC)NN↓NNNNNG(5mC)

PcsI

PctI

GAATGCN↑
CTTAC↓GN

BsmI

PkrI

Последовательность
ДНК,
содержащая не менее трех 5mC:
GCN↑GC
CG↓NCG

PkrI

Ple19I

CGAT↑CG
GC↓TAGC

PvuI

–

PpsI

GAGTC(N)4↑
CTCAG(N)5↓

PleI

PsiI

TTA↑TAA
AAT↓ATT

PsiI

Psp124BI

GAGCT↑C
C↓TCGAG

SacI

Psp6I

↑CCWGG
GGWCC↓

EcoRII

+

PspCI

CAC↑GTG
GTG↓CAC

PmaCI

PspEI

G↑GTNACC
CCANTG↓G

BstEII

PspLI

C↑GTACG
GCATG↓C

SplI

PspN4I

GGN↑NCC
CCN↓NGG

NlaIV

GGNN(m5C)C

PspOMI

G↑GGCCC
CCCGG↓G

(ApaI)

PspPPI

RG↑GWCCY
YCCWG↓GR

PpuMI

+

PspXI

VC↑TCGAGB
BGAGCT↓CV

PspXI

PsrI

↑(N)7GAACNNNNNNTAC(N)12↑
↓(N)12CTTGNNNNNNATG(N)7↓

PsrI

PstI

CTGCA↑G
G↓ACGTC

PstI

PstNI

CAGNNN↑CTG
GTC↓NNNGAC

AlwNI

PvuII

CAG↑CTG
GTC↓GAC

PvuII

RgaI

GCGAT↑CGC
CGC↓TAGCG

SgfI

–

RigI

GGCCGG↑CC
CC↓GGCCGG

FseI

GG(m5C)CGGCC

+

RsaI

GT↑AC
CA↓TG

RsaI

RsaNI

G↑TAC
CAT↓G

RsaI

Rsr2I

CG↑GWCCG
GCCWG↓GC

RsrII

SalI

G↑TCGAC
CAGCT↓G

SalI

SbfI

CCTGCA↑GG
GG↓ACGTCC

Sse8387I

SetI

ASST↑
↓TSSA

SetI

SfaNI

GCATC(N)5↑
CGTAG(N)9↓

SfaNI

SfiI

GGCCNNNN↑NGGCC
CCGGN↓NNNNCCGG

SfiI

+
GGC(m5C)WGGNNGGCC

Sfr274I

C↑TCGAG
GAGCT↓C

XhoI

CTCG(m6A)G

Sfr303I

CCGC↑GG
GG↓CGCC

SacII

SmaI

CCC↑GGG
GGG↓CCC

SmaI

SmiI

ATTT↑AAAT
TAAA↓TTTA

SwaI

SmiMI

CAYNN↑NNRTG
GTRNN↓NNYAC

MslI

SphI

GCATG↑C
C↓GTACG

SphI

Sse9I

↑AATT
TTAA↓

Tsp509I

A(m6A)TT

SspI

AAT↑ATT
TTA↓TAA

SspI

A(m6A)TATT

TaqI

T↑CGA
AGC↓T

TaqI

Tru9I

T↑TAA
AAT↓T

MseI

TTA(m6A)

TseFI

↑GTSAC
CASTG↓

Tsp45I

Tth111I

GACN↑NNGTC
CTGNN↓NCAG

Tth111I

VneI

G↑TGCAC
CACGT↓G

ApaLI

VspI

AT↑TAAT
TAAT↓TA

VspI

ATTA(m6A)T

XbaI

T↑CTAGA
AGATC↓T

XbaI

+

XmaI

C↑CCGGG
GGGCC↓C

XmaI

ZraI

GAC↑GTC
CTG↓CAG

(AatII)

ZrmI

AGT↑ACT
TCA↓TGA

ScaI

Zsp2I

ATGCA↑T
T↓ACGTA

NsiI