Активность эндонуклеаз рестрикции в различных SE-буферах и температура инактивации за 20 минут

Фермент Сайт узнавания Оптим. буфер BSA Активность (в % от максимальной) Оптимальная температура, °С Инактивация, °С
B G O W Y Rose
Aat II
GACGTC
CTGCAG
Y 10 – 25 25 – 50 10 – 25 25 – 50 100 50 37 65
Abs I
CCTCGAGG
GGAGCTCC
* 75 – 100 10 – 25 0 – 10 50 – 75 0 – 10 50 37 65
Acc16 I
TGCGCA
ACGCGT
W 50 – 75 75 – 100 25 – 50 100 75 – 100 70 37 65
Acc36 I
ACCTGC(N)4
TGGACG(N)8
Y 25 – 50 25 – 50 50 – 75 50 – 75 100 100 37 65
Acc65 I
GGTACC
CCATGG
W 10 – 25 25 – 50 75 – 100 100 10 – 25 100 37 65
AccB1 I
GGYRCC
CCRYGG
K + 50 – 75 10 – 25 10 – 25 75 – 100 50 – 75 30 37 65
AccB7 I
CCANNNNNTGG
GGTNNNNNACC
G 10 – 25 100 25 – 50 50 – 75 50 – 75 100 37 65
AccBS I
GAGCGG
CTCGCC
Y 75 – 100 75 – 100 25 – 50 25 – 50 100 100 37 65
Acl I
AACGTT
TTGCAA
Y + 0 – 10 0 – 10 0 – 10 0 – 10 100 80 37 65
AclW I
GGATC(N)4
CCTAG(N)5
Y + 75 – 100 50 – 75 0 – 10 0 – 10 100 30 37 65
Aco I
YGGCCR
RCCGGY
Y 50 – 75 75 – 100 50 – 75 25 – 50 100 100 37 65
Acs I
RAATTY
YTTAAR
W + 25 – 50 50 – 75 50 – 75 100 10 – 25 100 50 80
Acu I
CTGAAG(N)16
GACTTC(N)14
Y+ SAM + 25 – 50 50 – 75 50 – 75 75 – 100 100 50 37 65
Afe I
AGCGCT
TCGCGA
Y 10 – 25 25 – 50 75 – 100 75 – 100 100 100 37 65
Ags I
TTSAA
AASTT
Y + 75 – 100 50 – 75 10 – 25 10 – 25 100 50 37 65
Ahl I
ACTAGT
TGATCA
B + 100 75 – 100 25 – 50 25 – 50 75 – 100 100 37 N
Ajn I
CCWGG
GGWCC
Y 25 – 50 10 – 25 10 – 25 25 – 50 100 50 55 65
Alu I
AGCT
TCGA
Y 75 – 100 75 – 100 10 – 25 50 – 75 100 50 37 65
AluB I
AGCT
TCGA
B + 100 75 – 100 10 – 25 10 – 25 75 – 100 60 37 65
Ama87 I
CYCGRG
GRGCYC
W + 10 – 25 50 – 75 75 – 100 100 0 – 10 25 37 65
Apa I
GGGCCC
CCCGGG
Y + 50 – 75 25 – 50 0 – 10 0 – 10 100 50 37 65
Ars I
(N)8GACNNNNNNTTYG(N)11
(N)13CTGNNNNNNAARC(N)6
Y + 0 – 10 0 – 10 0 – 10 0 – 10 100 20 30 65
AsiG I
ACCGGT
TGGCCA
O 10 – 25 25 – 50 100 75 – 100 10 – 25 75 37 65
AsiS I
GCGATCGC
CGCTAGCG
B 100 75 – 100 0 – 10 10 – 25 25 – 50 25 37 80
AspA2 I
CCTAGG
GGATCC
W + 10 – 25 50 – 75 75 – 100 100 75 – 100 10 37 80
AspLE I
GCGC
CGCG
O 10 – 25 75 – 100 100 50 – 75 25 – 50 100 37 N
AspS9 I
GGNCC
CCNGG
W 50 – 75 50 – 75 75 – 100 100 50 – 75 75 37 65
AsuC2 I
CCSGG
GGSCC
Y 75 – 100 50 – 75 10 – 25 25 – 50 100 40 37 65
AsuHP I
GGTGA(N)8
CCACT(N)7
O 10 – 25 50 – 75 100 75 – 100 25 – 50 100 37 65
AsuNH I
GCTAGC
CGATCG
Y + 75 – 100 50 – 75 0 – 10 0 – 10 100 25 37 65
BamH I
GGATCC
CCTAGG
G + 25 – 50 100 75 – 100 75 – 100 25 – 50 100 37 65
Bar I
(N)7GAAGNNNNNNTAC(N)12
(N)12CTTCNNNNNNATG(N)7
* 0 – 10 0 – 10 25 – 50 50 – 75 10 – 25 40 37 65
Bbv12 I
GWGCWC
CWCGWG
O 0 – 10 10 – 25 100 75 – 100 10 – 25 60 37 80
Bgl I
GCCNNNNNGGC
CGGNNNNNCCG
2W 50 – 75 50 – 75 0 – 10 75 – 100 25 – 50 100 37 65
Bgl II
AGATCT
TCTAGA
O 0 – 10 10 – 25 100 25 – 50 10 – 25 100 37 80
Bme18 I
GGWCC
CCWGG
O 10 – 25 25 – 50 100 75 – 100 10 – 25 80 37 65
Bmt I
GCTAGC
CGATCG
W 10 – 25 50 – 75 50 – 75 100 75 – 100 100 37 65
Bmu I
ACTGGG(N)5
TGACCC(N)4
Y 75 – 100 75 – 100 25 – 50 10 – 25 100 25 37 65
Bpm I
CTGGAG(N)16
GACCTC(N)14
W + 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
Bpu10 I
CCTNAGC
GGANTCG
K 10 – 25 25 – 50 50 – 75 50 – 75 25 – 50 100 37 80
Bpu14 I
TTCGAA
AAGCTT
G 50 – 75 100 25 – 50 25 – 50 75 – 100 100 37 65
Bsa29 I
ATCGAT
TAGCTA
G + 25 – 50 100 50 – 75 50 – 75 75 – 100 100 37 65
Bsc4 I
CCNNNNNNNGG
GGNNNNNNNCC
W + 75 – 100 75 – 100 50 – 75 100 25 – 50 90 55 80
Bse1 I
ACTGGN
TGACCN
Y 75 – 100 75 – 100 25 – 50 10 – 25 100 100 65 80
Bse118 I
RCCGGY
YGGCCR
O 0 – 10 50 – 75 100 75 – 100 25 – 50 100 65 80
Bse21 I
CCTNAGG
GGANTCC
Y 50 – 75 50 – 75 10 – 25 25 – 50 100 40 37 80
Bse3D I
GCAATGNN
CGTTACNN
G 10 – 25 100 25 – 50 50 – 75 75 – 100 100 60 80
Bse8 I
GATNNNNATC
CTANNNNTAG
G 25 – 50 100 75 – 100 75 – 100 50 – 75 100 60 80
BseP I
GCGCGC
CGCGCG
G 50 – 75 100 75 – 100 50 – 75 50 – 75 100 50 65
BseX3 I
CGGCCG
GCCGGC
O 10 – 25 25 – 50 100 50 – 75 10 – 25 25 50 80
BslF I
GGGAC(N)10
CCCTG(N)14
Y + 25 – 50 25 – 50 10 – 25 25 – 50 100 50 37 80
Bso31 I
GGTCTCN
CCAGAG(N)5
O + 25 – 50 75 – 100 100 75 – 100 25 – 50 40 55 80
Bsp13 I
TCCGGA
AGGCCT
2K 25 – 50 50 – 75 75 – 100 50 – 75 0 – 10 5 50 65
Bsp1720 I
GCTNAGC
CGANTCG
G 50 – 75 100 50 – 75 50 – 75 75 – 100 75 37 80
Bsp19 I
CCATGG
GGTACC
2W + 0 – 10 10 – 25 50 – 75 75 – 100 10 – 25 5 37 65
BspAC I
CCGC
GGCG
O + 10 – 25 25 – 50 100 75 – 100 10 – 25 100 37 65
BspFN I
CGCG
GCGC
Y 50 – 75 75 – 100 75 – 100 50 – 75 100 100 37 65
BssEC I
CCNNGG
GGNNCC
Y 50 – 75 50 – 75 50 – 75 75 – 100 100 100 60 80
BssNA I
GTATAC
CATATG
W + 50 – 75 50 – 75 75 – 100 100 75 – 100 100 37 N
BssT1 I
CCWWGG
GGWWCC
2K 10 – 25 25 – 50 25 – 50 75 – 100 10 – 25 100 60 80
Bst2B I
CTCGTG
GAGCAC
Y + 75 – 100 25 – 50 10 – 25 25 – 50 100 100 60 80
Bst2U I
CCWGG
GGWCC
G + 75 – 100 100 50 – 75 50 – 75 10 – 25 50 60 N
Bst4C I
ACNGT
TGNCA
Y 75 – 100 75 – 100 10 – 25 25 – 50 100 50 65 N
Bst6 I
CTCTTCN
GAGAAG(N)4
Y + 75 – 100 75 – 100 50 – 75 75 – 100 100 100 65 80
BstAC I
GRCGYC
CYGCRG
W 75 – 100 75 – 100 50 – 75 100 75 – 100 100 37 80
BstAF I
CTTAAG
GAATTC
W + 10 – 25 25 – 50 75 – 100 100 25 – 50 100 55 80
BstAP I
GCANNNNNTGC
CGTNNNNNACG
W 25 – 50 25 – 50 75 – 100 100 25 – 50 100 60 80
BstAU I
TGTACA
ACATGT
W 10 – 25 50 – 75 25 – 50 100 25 – 50 100 37 80
BstBA I
YACGTR
RTGCAY
W + 25 – 50 25 – 50 75 – 100 100 25 – 50 50 65 80
BstC8 I
GCNNGC
CGNNCG
Y 10 – 25 25 – 50 50 – 75 75 – 100 100 100 55 80
BstDE I
CTNAG
GANTC
G 75 – 100 100 25 – 50 50 – 75 10 – 25 100 60 80
BstDS I
CCRYGG
GGYRCC
Y 0 – 10 75 – 100 50 – 75 25 – 50 100 100 65 80
BstEN I
CCTNNNNNAGG
GGANNNNNTCC
Y 50 – 75 50 – 75 25 – 50 25 – 50 100 100 65 80
BstF5 I
GGATGNN
CCTACNN
Y 75 – 100 50 – 75 25 – 50 50 – 75 100 100 65 80
BstFN I
CGCG
GCGC
Y 75 – 100 50 – 75 25 – 50 25 – 50 100 75 60 80
BstH2 I
RGCGCY
YCGCGR
Y + 50 – 75 50 – 75 0 – 10 10 – 25 100 100 65 80
BstHH I
GCGC
CGCG
Y + 75 – 100 50 – 75 25 – 50 50 – 75 100 100 50 N
BstKT I
GATC
CTAG
W 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
BstMA I
GTCTCN
CAGAG(N)5
W + 25 – 50 50 – 75 50 – 75 100 75 – 100 100 55 65
BstMB I
GATC
CTAG
O 10 – 25 25 – 50 100 75 – 100 10 – 25 100 65 80
BstMC I
CGRYCG
GCYRGC
B + 100 75 – 100 10 – 25 10 – 25 50 – 75 100 50 80
BstMW I
GCNNNNNNNGC
CGNNNNNNNCG
Y 10 – 25 25 – 50 25 – 50 50 – 75 100 40 55 80
BstNS I
RCATGY
YGTACR
B + 100 50 – 75 10 – 25 10 – 25 75 – 100 50 37 65
BstPA I
GACNNNNGTC
CTGNNNNCAG
Y 50 – 75 25 – 50 50 – 75 50 – 75 100 100 65 N
BstSC I
CCNGG
GGNCC
Y 50 – 75 50 – 75 50 – 75 50 – 75 100 60 55 80
BstSF I
CTRYAG
GAYRTC
O + 75 – 100 25 – 50 100 50 – 75 50 – 75 100 60 N
BstSL I
GKGCMC
CMCGKG
G + 50 – 75 100 50 – 75 75 – 100 75 – 100 100 55 65
BstSN I
TACGTA
ATGCAT
B 100 50 – 75 0 – 10 10 – 25 50 – 75 50 37 80
BstV1 I
GCAGC(N)8
CGTCG(N)12
G 75 – 100 100 75 – 100 75 – 100 75 – 100 100 55 80
BstV2 I
GAAGAC(N)2
CTTCTG(N)6
Y + 75 – 100 75 – 100 25 – 50 25 – 50 100 70 55 65
BstX I
CCANNNNNNTGG
GGTNNNNNNACC
O 10 – 25 10 – 25 100 75 – 100 25 – 50 100 37 65
BstX2 I
RGATCY
YCTAGR
G 75 – 100 100 0 – 10 10 – 25 25 – 50 100 60 80
Bsu I
GTATCC(N)6
CATAGG(N)5
Y 75 – 100 50 – 75 10 – 25 25 – 50 100 10 37 65
BsuR I
GGCC
CCGG
G 75 – 100 100 25 – 50 50 – 75 50 – 75 100 37 80
Btr I
CACGTC
GTGCAG
O + 75 – 100 75 – 100 100 75 – 100 75 – 100 100 60 80
Cci I
TCATGA
AGTACT
W + 0 – 10 10 – 25 25 – 50 100 75 – 100 100 55 80
CciN I
GCGGCCGC
CGCCGGCG
Y 25 – 50 50 – 75 75 – 100 75 – 100 100 100 37 65
Dra I
TTTAAA
AAATTT
G + 75 – 100 100 25 – 50 75 – 100 75 – 100 100 37 65
Dra III
CACNNNGTG
GTGNNNCAC
2K + 25 – 50 50 – 75 75 – 100 75 – 100 50 – 75 100 37 65
Dri I
GACNNNNNGTC
CTGNNNNNCAG
Y 75 – 100 75 – 100 10 – 25 10 – 25 100 40 37 65
DseD I
GACNNNNNNGTC
CTGNNNNNNCAG
Y + 75 – 100 75 – 100 25 – 50 50 – 75 100 30 37 80
EcoICR I
GAGCTC
CTCGAG
G + 75 – 100 100 0 – 10 0 – 10 75 – 100 5 37 65
EcoR I
GAATTC
CTTAAG
* + 50 – 75 75 – 100 75 – 100 75 – 100 50 – 75 50 37 65
EcoR V
GATATC
CTATAG
W + 0 – 10 25 – 50 50 – 75 100 25 – 50 50 37 80
Ege I
GGCGCC
CCGCGG
B + 100 75 – 100 10 – 25 50 – 75 75 – 100 100 37 65
Erh I
CCWWGG
GGWWCC
2W + 10 – 25 25 – 50 50 – 75 75 – 100 10 – 25 100 37 65
Fae I
CATG
GTAC
* + 25 – 50 50 – 75 10 – 25 10 – 25 75 – 100 100 37 65
Fai I
YATR
RTAY
B 100 50 – 75 10 – 25 25 – 50 25 – 50 100 50 80
Fal I
(N)8AAGNNNNNCTT(N)13
(N)13TTCNNNNNGAA(N)8
W+ SAM 0 – 10 25 – 50 75 – 100 100 50 – 75 70 37 65
Fat I
CATG
GTAC
G 10 – 25 100 25 – 50 10 – 25 50 – 75 100 55 65
Fau I
CCCGC(N)4
GGGCG(N)6
B 100 25 – 50 0 – 10 0 – 10 50 – 75 50 55 65
FauND I
CATATG
GTATAC
Y + 50 – 75 75 – 100 10 – 25 50 – 75 100 100 37 65
Fbl I
GTMKAC
CAKMTG
Y 50 – 75 75 – 100 0 – 10 50 – 75 100 100 55 80
Fok I
GGATG(N)9
CCTAC(N)13
Y 50 – 75 50 – 75 25 – 50 25 – 50 100 100 37 65
FriO I
GRGCYC
CYCGRG
Y + 75 – 100 75 – 100 10 – 25 0 – 10 100 25 37 65
Fsp4H I
GCNGC
CGNCG
Y 50 – 75 75 – 100 10 – 25 25 – 50 100 100 37 65
Gsa I
CCCAGC
GGGTCG
W + 10 – 25 25 – 50 75 – 100 100 75 – 100 100 70 N
Hae III
GGCC
CCGG
G 75 – 100 100 25 – 50 50 – 75 50 – 75 100 37 80
Hga I
GACGC(N)5
CTGCG(N)10
B 100 75 – 100 10 – 25 25 – 50 50 – 75 50 37 65
Hind II
GTYRAC
CARYTG
G + 75 – 100 100 25 – 50 25 – 50 75 – 100 50 37 65
Hind III
AAGCTT
TTCGAA
W + 10 – 25 25 – 50 0 – 10 100 0 – 10 100 37 80
Hinf I
GANTC
CTNAG
O 25 – 50 75 – 100 100 75 – 100 75 – 100 100 37 80
Hpa I
GTTAAC
CAATTG
Y 0 – 10 50 – 75 10 – 25 25 – 50 100 25 37 65
Hpa II
CCGG
GGCC
B 100 50 – 75 10 – 25 25 – 50 50 – 75 50 37 80
HpySE526 I
ACGT
TGCA
Y 75 – 100 75 – 100 10 – 25 25 – 50 100 50 37 65
HspA I
GCGC
CGCG
Y 50 – 75 50 – 75 25 – 50 25 – 50 100 100 37 80
Kpn I
GGTACC
CCATGG
B + 100 25 – 50 25 – 50 25 – 50 75 – 100 50 37 80
Ksp22 I
TGATCA
ACTAGT
2K + 50 – 75 75 – 100 50 – 75 50 – 75 25 – 50 100 37 65
Kzo9 I
GATC
CTAG
G 50 – 75 100 50 – 75 50 – 75 50 – 75 100 37 65
Lmn I
GCTCCN
CGAGGN
B 100 75 – 100 50 – 75 50 – 75 75 – 100 80 37 65
Mab I
ACCWGGT
TGGWCCA
W + 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
Mbo II
GAAGA(N)8
CTTCT(N)7
Y 75 – 100 75 – 100 25 – 50 50 – 75 100 50 37 65
Mfe I
CAATTG
GTTAAC
B + 100 75 – 100 10 – 25 25 – 50 75 – 100 25 37 N
Mhl I
GDGCHC
CHCGDG
W 10 – 25 25 – 50 75 – 100 100 10 – 25 100 37 80
Mlu I
ACGCGT
TGCGCA
O 0 – 10 10 – 25 100 25 – 50 10 – 25 50 37 65
Mly113 I
GGCGCC
CCGCGG
B 100 25 – 50 10 – 25 10 – 25 50 – 75 100 37 65
Mnl I
CCTC(N)7
GGAG(N)6
G + 75 – 100 100 25 – 50 25 – 50 75 – 100 100 37 65
Mox20 I
TGGCCA
ACCGGT
O 10 – 25 25 – 50 100 75 – 100 25 – 50 75 37 N
MroN I
GCCGGC
CGGCCG
B 100 50 – 75 10 – 25 0 – 10 10 – 25 5 37 80
MroX I
GAANNNNTTC
CTTNNNNAAG
W 50 – 75 50 – 75 50 – 75 100 25 – 50 100 37 65
Msp I
CCGG
GGCC
B 100 75 – 100 50 – 75 75 – 100 75 – 100 100 37 65
MspA1 I
CMGCKG
GKCGMC
Y + 10 – 25 75 – 100 10 – 25 25 – 50 100 100 37 65
MspR9 I
CCNGG
GGNCC
O 50 – 75 50 – 75 100 50 – 75 50 – 75 100 37 80
Nru I
TCGCGA
AGCGCT
W 0 – 10 10 – 25 75 – 100 100 10 – 25 100 37 80
PalA I
GGCGCGCC
CCGCGCGG
Y 25 – 50 10 – 25 75 – 100 100 100 40 37 65
Pce I
AGGCCT
TCCGGA
Y 75 – 100 75 – 100 50 – 75 25 – 50 100 100 50 80
Pci I
ACATGT
TGTACA
O 50 – 75 75 – 100 100 75 – 100 50 – 75 50 37 65
PciS I
GCTCTTCN
CGAGAAG(N)4
B 100 50 – 75 0 – 10 0 – 10 75 – 100 50 37 65
Pct I
GAATGCN
CTTACGN
O 25 – 50 50 – 75 100 75 – 100 10 – 25 50 37 65
Ple19 I
CGATCG
GCTAGC
Y 75 – 100 75 – 100 25 – 50 25 – 50 100 100 37 65
Pps I
GAGTC(N)4
CTCAG(N)5
Y + 50 – 75 10 – 25 0 – 10 25 – 50 100 50 37 65
Psi I
TTATAA
AATATT
B 100 25 – 50 10 – 25 25 – 50 75 – 100 40 37 65
Psp124B I
GAGCTC
CTCGAG
G 75 – 100 100 10 – 25 0 – 10 75 – 100 30 37 80
Psp6 I
CCWGG
GGWCC
B 100 50 – 75 10 – 25 25 – 50 75 – 100 10 55 80
PspC I
CACGTG
GTGCAC
B + 100 50 – 75 0 – 10 0 – 10 50 – 75 5 37 65
PspE I
GGTNACC
CCANTGG
B 100 50 – 75 25 – 50 50 – 75 50 – 75 100 37 65
PspL I
CGTACG
GCATGC
Y + 75 – 100 75 – 100 25 – 50 10 – 25 100 100 37 65
PspN4 I
GGNNCC
CCNNGG
Y 10 – 25 10 – 25 10 – 25 25 – 50 100 50 37 65
PspOM I
GGGCCC
CCCGGG
Y 75 – 100 10 – 25 0 – 10 0 – 10 100 25 37 65
PspPP I
RGGWCCY
YCCWGGR
Y + 10 – 25 10 – 25 0 – 10 0 – 10 100 100 37 65
PspX I
VCTCGAGB
BGAGCTCV
Y + 50 – 75 50 – 75 25 – 50 75 – 100 100 25 37 80
Psr I
(N)7GAACNNNNNNTAC(N)12
(N)12CTTGNNNNNNATG(N)7
Y + 10 – 25 10 – 25 0 – 10 0 – 10 100 30 30 65
Pst I
CTGCAG
GACGTC
O + 10 – 25 25 – 50 100 25 – 50 25 – 50 50 37 80
PstN I
CAGNNNCTG
GTCNNNGAC
Y 25 – 50 50 – 75 10 – 25 25 – 50 100 100 37 65
Pvu II
CAGCTG
GTCGAC
G + 25 – 50 100 25 – 50 25 – 50 25 – 50 100 37 80
Rga I
GCGATCGC
CGCTAGCG
Y 75 – 100 50 – 75 10 – 25 25 – 50 100 100 55 80
Rig I
GGCCGGCC
CCGGCCGG
* + 75 – 100 50 – 75 0 – 10 10 – 25 50 – 75 10 37 65
Rsa I
GTAC
CATG
B 100 50 – 75 0 – 10 50 – 75 75 – 100 50 37 N
RsaN I
GTAC
CATG
B 100 75 – 100 50 – 75 50 – 75 75 – 100 100 37 80
Rsr2 I
CGGWCCG
GCCWGGC
Y + 50 – 75 75 – 100 0 – 10 10 – 25 100 25 37 65
Sal I
GTCGAC
CAGCTG
O 0 – 10 10 – 25 100 25 – 50 0 – 10 5 37 65
Sbf I
CCTGCAGG
GGACGTCC
Y 75 – 100 50 – 75 0 – 10 0 – 10 100 50 37 80
Set I
ASST
TSSA
Y 25 – 50 25 – 50 75 – 100 75 – 100 100 100 55 80
SfaN I
GCATC(N)5
CGTAG(N)9
O 10 – 25 25 – 50 100 75 – 100 0 – 10 25 37 80
Sfi I
GGCCNNNNNGGCC
CCGGNNNNNCCGG
G + 75 – 100 100 25 – 50 25 – 50 25 – 50 75 50 65
Sfr274 I
CTCGAG
GAGCTC
B 100 75 – 100 50 – 75 50 – 75 75 – 100 100 50 65
Sfr303 I
CCGCGG
GGCGCC
B 100 50 – 75 10 – 25 10 – 25 75 – 100 100 37 65
Sma I
CCCGGG
GGGCCC
Y 0 – 10 0 – 10 0 – 10 0 – 10 100 50 25 65
Smi I
ATTTAAAT
TAAATTTA
O + 25 – 50 25 – 50 100 75 – 100 25 – 50 10 37 65
SmiM I
CAYNNNNRTG
GTRNNNNYAC
W 10 – 25 10 – 25 75 – 100 100 10 – 25 60 37 65
Sph I
GCATGC
CGTACG
G + 25 – 50 100 75 – 100 75 – 100 50 – 75 100 37 65
Sse9 I
AATT
TTAA
B + 100 75 – 100 50 – 75 50 – 75 75 – 100 75 55 65
Ssp I
AATATT
TTATAA
K + 75 – 100 50 – 75 25 – 50 50 – 75 75 – 100 100 37 65
Taq I
TCGA
AGCT
Y + 50 – 75 75 – 100 75 – 100 50 – 75 100 100 65 80
Tru9 I
TTAA
AATT
W 75 – 100 25 – 50 25 – 50 100 50 – 75 100 65 80
TseF I
GTSAC
CASTG
B 100 50 – 75 0 – 10 25 – 50 50 – 75 50 65 N
Tth111 I
GACNNNGTC
CTGNNNCAG
Y 75 – 100 50 – 75 10 – 25 10 – 25 100 100 65 80
Vne I
GTGCAC
CACGTG
O 10 – 25 25 – 50 100 25 – 50 25 – 50 100 37 65
Vsp I
ATTAAT
TAATTA
W 0 – 10 10 – 25 50 – 75 100 25 – 50 50 37 65
Xba I
TCTAGA
AGATCT
O + 75 – 100 75 – 100 100 50 – 75 75 – 100 25 37 65
Xma I
CCCGGG
GGGCCC
Y 75 – 100 50 – 75 0 – 10 0 – 10 100 50 37 65
Zra I
GACGTC
CTGCAG
B 100 50 – 75 25 – 50 25 – 50 75 – 100 100 37 80
Zrm I
AGTACT
TCATGA
Y + 50 – 75 25 – 50 0 – 10 0 – 10 100 50 37 65
Zsp2 I
ATGCAT
TACGTA
B + 100 50 – 75 25 – 50 25 – 50 25 – 50 50 37 65