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Optimal buffers contents


BufferContent
  SE-buffer B  10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.
  SE-buffer G  10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.
  SE-buffer O  50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.
  SE-buffer W  10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.
  SE-buffer Y  33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.
  SE-buffer K  10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.
  SE-buffer 2K  10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.
  SE-buffer AbsI  10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.
  SE-buffer AoxI  10 mM Tris-HCl (pH 7,5 at 25°C); 200 mM KCl; 0,1 mM EDTA, 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol.
  SE-buffer BisI  10 mM Tris-HCl (pH 9,0 at 25°C); 10 mM MgCl2; 150 mM KCl; 1 mM DTT.
  SE-buffer EcoRI  100 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.
  SE-buffer FaeI  33 mM Tris-acetate (pH 8.3 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.
  SE-buffer GLAD  50 mM Tris-SO4, pH 9.0 at 25°C; 30 mM KCl; 10 mM [NH4]2SO4
  SE-buffer MalI  20 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.
  SE-buffer N.Bst9I  10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 150 mM KCl; 1 mM DTT.
  SE-buffer PcsI  10 mM Tris-HCl (pH 8.3 at 25°C); 20mM NaCl, 3 mM MgCl2, 1 mM DTT.
  SE-buffer RigI  10 mM Tris-HCl (pH 8.5 at 25°C); 5 mM MgCl2; 1mM DTT.
  SE-buffer polynucleotide kinase T4  50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 5 mM DTT.
  SE-buffer DNA ligase T4  50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.
  SE-buffer Klenow fragment  50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 5 mM DTT.
  SE-buffer AS (Ammonium Sulfate)  67 mM Tris-HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2.
  SE-buffer DNA polymerase Taq  60 mM Tris-HCl (pH 8.5 at 25°C); 1.5 mM MgCl2; 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.
  SE-buffer DNA polymerase Taq (Mg2+ free)  60 mM Tris-HCl (pH 8.5 at 25°C); 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.
  SE-buffer DNA polymerase T4  67 mM Tris-HCl (pH 8.8 at 25°C); 6.7 mM MgCl2;
16.7 mM (NH4)2SO4, 1 mM DTT.
  SE-buffer RNA polymerase T7  50 mM Tris-HCl (pH 7.5 at 25°C); 6 mM MgCl2; 10 mM Dithiothreitol; 2 mM spermidine.
  SE-buffer Reverse transcriptase M-MuLV  50 mM Tris-HCl (pH 8.3 at 25°C); 3 mM MgCl2; 75 mM KCl; 10 mM DTT.
  SE-buffer Reverse transcriptase M-MuLV dilution  10 mM KH2PO4(pH 7.5); 0,1 mM EDTA; 200 mM NaCl; 7 mM 2-mercaptoethanol; 50% glycerol
  SE-buffer Inorganic pyrophosphatase  50 mM Tris-HCl (pH 8.5 at 25°C); 1 mM MgCl2.
  SE-buffer Exonuclease III  50 mM Tris-HCl (pH 7.6 at 25°C); 1 mM MgCl2.
  SE-buffer Uracil DNA Glycosylase  20 mM Tris-HCl (pH 8.0 at 25°C); 1 mM EDTA; 1 mM DTT
  SE-buffer 2W  20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT
  SE-buffer Endonuclease I  20 mM Glycine-NaOH (pH 9.5 at 25°C); 25 mM MgCl2; 100mM NaCl; 1mM 2-mercaptoethanol
  SE-buffer Alkaline phosphatase  50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.
  SE-buffer RNA ligase T4  50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.
  SE-buffer Hot Start Taq DNA polymerase  67 mM Tris-HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2.
  SE-buffer Pfu DNA polymerase  20 mM Tris-HCl (pH 8.8 at 25°C); 10 mM KCl, 10 mM (NH4)2SO4, 2 mM MgSO4, 0.1 % Triton X-100 )+ 100 μg/ml BSA
  SE-buffer A (Storage and dilution)  10 mM Tris-HCl (pH 7.6 at 25°C); 50 mM KCl; 0,1 mM EDTA; 200 mkg/ml BSA; 1mM DTT; 50% glycerol.
  Loading buffer
for DNA Ladders
  0.01% bromophenol blue; 0.25M EDTA; 50% glycerol.

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