Активность эндонуклеаз рестрикции в различных SE-буферах и температура инактивации за 20 минут
| Фермент | Сайт узнавания | Оптим. буфер | BSA | Активность (в % от максимальной) | Оптимальная температура, °С | Инактивация, °С | |||||
| B | G | O | W | Y | Rose | ||||||
| Aat II | C↓TGCAG |
Y | — | 10 — 25 | 25 — 50 | 10 — 25 | 25 — 50 | 100 | 50 | 37 | 65 |
| Abs I | GGAGCT↓CC |
* | — | 75 — 100 | 10 — 25 | 0 — 10 | 50 — 75 | 0 — 10 | 50 | 37 | 65 |
| Acc16 I | ACG↓CGT |
W | — | 50 — 75 | 75 — 100 | 25 — 50 | 100 | 75 — 100 | 70 | 37 | 65 |
| Acc36 I | TGGACG(N)8↓ |
Y | — | 25 — 50 | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 100 | 37 | 65 |
| Acc65 I | CCATG↓G |
W | — | 10 — 25 | 25 — 50 | 75 — 100 | 100 | 10 — 25 | 100 | 37 | 65 |
| AccB1 I | CCRYG↓G |
K | + | 50 — 75 | 10 — 25 | 10 — 25 | 75 — 100 | 50 — 75 | 30 | 37 | 65 |
| AccB7 I | GGTN↓NNNNACC |
G | — | 10 — 25 | 100 | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 37 | 65 |
| AccBS I | CTC↓GCC |
Y | — | 75 — 100 | 75 — 100 | 25 — 50 | 25 — 50 | 100 | 100 | 37 | 65 |
| Acl I | TTGC↓AA |
Y | + | 0 — 10 | 0 — 10 | 0 — 10 | 0 — 10 | 100 | 80 | 37 | 65 |
| AclW I | CCTAG(N)5↓ |
Y | + | 75 — 100 | 50 — 75 | 0 — 10 | 0 — 10 | 100 | 30 | 37 | 65 |
| Aco I | RCCGG↓Y |
Y | — | 50 — 75 | 75 — 100 | 50 — 75 | 25 — 50 | 100 | 100 | 37 | 65 |
| Acs I | YTTAA↓R |
W | + | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 10 — 25 | 100 | 50 | 80 |
| Acu I | GACTTC(N)14↓ |
Y+ SAM | + | 25 — 50 | 50 — 75 | 50 — 75 | 75 — 100 | 100 | 50 | 37 | 65 |
| Afe I | TCG↓CGA |
Y | — | 10 — 25 | 25 — 50 | 75 — 100 | 75 — 100 | 100 | 100 | 37 | 65 |
| Ags I | AA↓STT |
Y | + | 75 — 100 | 50 — 75 | 10 — 25 | 10 — 25 | 100 | 50 | 37 | 65 |
| Ahl I | TGATC↓A |
B | + | 100 | 75 — 100 | 25 — 50 | 25 — 50 | 75 — 100 | 100 | 37 | N |
| Ajn I | GGWCC↓ |
Y | — | 25 — 50 | 10 — 25 | 10 — 25 | 25 — 50 | 100 | 50 | 55 | 65 |
| Alu I | TC↓GA |
Y | — | 75 — 100 | 75 — 100 | 10 — 25 | 50 — 75 | 100 | 50 | 37 | 65 |
| AluB I | TC↓GA |
B | + | 100 | 75 — 100 | 10 — 25 | 10 — 25 | 75 — 100 | 60 | 37 | 65 |
| Ama87 I | GRGCY↓C |
W | + | 10 — 25 | 50 — 75 | 75 — 100 | 100 | 0 — 10 | 25 | 37 | 65 |
| Apa I | C↓CCGGG |
Y | + | 50 — 75 | 25 — 50 | 0 — 10 | 0 — 10 | 100 | 50 | 37 | 65 |
| Ars I | ↓(N)13CTGNNNNNNAARC(N)6↓ |
Y | + | 0 — 10 | 0 — 10 | 0 — 10 | 0 — 10 | 100 | 20 | 30 | 65 |
| AsiG I | TGGCC↓A |
O | — | 10 — 25 | 25 — 50 | 100 | 75 — 100 | 10 — 25 | 75 | 37 | 65 |
| AspA2 I | GGATC↓C |
W | + | 10 — 25 | 50 — 75 | 75 — 100 | 100 | 75 — 100 | 10 | 37 | 80 |
| AspLE I | C↓GCG |
O | — | 10 — 25 | 75 — 100 | 100 | 50 — 75 | 25 — 50 | 100 | 37 | N |
| AspS9 I | CCNG↓G |
W | — | 50 — 75 | 50 — 75 | 75 — 100 | 100 | 50 — 75 | 75 | 37 | 65 |
| AsuC2 I | GGS↓CC |
Y | — | 75 — 100 | 50 — 75 | 10 — 25 | 25 — 50 | 100 | 40 | 37 | 65 |
| AsuHP I | CCACT(N)7↓ |
O | — | 10 — 25 | 50 — 75 | 100 | 75 — 100 | 25 — 50 | 100 | 37 | 65 |
| AsuNH I | CGATC↓G |
Y | + | 75 — 100 | 50 — 75 | 0 — 10 | 0 — 10 | 100 | 25 | 37 | 65 |
| BamH I | CCTAG↓G |
G | + | 25 — 50 | 100 | 75 — 100 | 75 — 100 | 25 — 50 | 100 | 37 | 65 |
| Bar I | ↓(N)12CTTCNNNNNNATG(N)7↓ |
* | — | 0 — 10 | 0 — 10 | 25 — 50 | 50 — 75 | 10 — 25 | 40 | 37 | 65 |
| Bbv12 I | C↓WCGWG |
O | — | 0 — 10 | 10 — 25 | 100 | 75 — 100 | 10 — 25 | 60 | 37 | 80 |
| Bgl I | CGGN↓NNNNCCG |
2W | — | 50 — 75 | 50 — 75 | 0 — 10 | 75 — 100 | 25 — 50 | 100 | 37 | 65 |
| Bgl II | TCTAG↓A |
O | — | 0 — 10 | 10 — 25 | 100 | 25 — 50 | 10 — 25 | 100 | 37 | 80 |
| Bme18 I | CCWG↓G |
O | — | 10 — 25 | 25 — 50 | 100 | 75 — 100 | 10 — 25 | 80 | 37 | 65 |
| Bmt I | C↓GATCG |
W | — | 10 — 25 | 50 — 75 | 50 — 75 | 100 | 75 — 100 | 100 | 37 | 65 |
| Bmu I | TGACCC(N)4↓ |
Y | — | 75 — 100 | 75 — 100 | 25 — 50 | 10 — 25 | 100 | 25 | 37 | 65 |
| BpmI | GACCTC(N)14↓ |
W | + | 25 — 50 | 50 — 75 | 75 — 100 | 100 | 50 — 75 | 100 | 37 | 65 |
| Bpu10I | GGANT↓CG |
K | — | 10 — 25 | 25 — 50 | 50 — 75 | 50 — 75 | 25 — 50 | 100 | 37 | 80 |
| Bpu14 I | AAGC↓TT |
G | — | 50 — 75 | 100 | 25 — 50 | 25 — 50 | 75 — 100 | 100 | 37 | 65 |
| Bsa29 I | TAGC↓TA |
G | + | 25 — 50 | 100 | 50 — 75 | 50 — 75 | 75 — 100 | 100 | 37 | 65 |
| Bsc4 I | GGNN↓NNNNNCC |
W | + | 75 — 100 | 75 — 100 | 50 — 75 | 100 | 25 — 50 | 90 | 55 | 80 |
| Bse1 I | TGAC↓CN |
Y | — | 75 — 100 | 75 — 100 | 25 — 50 | 10 — 25 | 100 | 100 | 65 | 80 |
| Bse118 I | YGGCC↓R |
O | — | 0 — 10 | 50 — 75 | 100 | 75 — 100 | 25 — 50 | 100 | 65 | 80 |
| Bse21 I | GGANT↓CC |
Y | — | 50 — 75 | 50 — 75 | 10 — 25 | 25 — 50 | 100 | 40 | 37 | 80 |
| Bse3D I | CGTTAC↓NN |
G | — | 10 — 25 | 100 | 25 — 50 | 50 — 75 | 75 — 100 | 100 | 60 | 80 |
| Bse8 I | CTANN↓NNTAG |
G | — | 25 — 50 | 100 | 75 — 100 | 75 — 100 | 50 — 75 | 100 | 60 | 80 |
| BseP I | CGCGC↓G |
G | — | 50 — 75 | 100 | 75 — 100 | 50 — 75 | 50 — 75 | 100 | 50 | 65 |
| BseX3 I | GCCGG↓C |
O | — | 10 — 25 | 25 — 50 | 100 | 50 — 75 | 10 — 25 | 25 | 50 | 80 |
| BslF I | CCCTG(N)14↓ |
Y | + | 25 — 50 | 25 — 50 | 10 — 25 | 25 — 50 | 100 | 50 | 37 | 80 |
| Bso31 I | CCAGAG(N)5↓ |
O | + | 25 — 50 | 75 — 100 | 100 | 75 — 100 | 25 — 50 | 40 | 55 | 80 |
| Bsp13 I | AGGCC↓T |
2K | — | 25 — 50 | 50 — 75 | 75 — 100 | 50 — 75 | 0 — 10 | 5 | 50 | 65 |
| Bsp1720 I | CGANT↓CG |
G | — | 50 — 75 | 100 | 50 — 75 | 50 — 75 | 75 — 100 | 75 | 37 | 80 |
| Bsp19 I | GGTAC↓C |
2W | + | 0 — 10 | 10 — 25 | 50 — 75 | 75 — 100 | 10 — 25 | 5 | 37 | 65 |
| BspAC I | GGC↓G |
O | + | 10 — 25 | 25 — 50 | 100 | 75 — 100 | 10 — 25 | 100 | 37 | 65 |
| BspFN I | GC↓GC |
Y | — | 50 — 75 | 75 — 100 | 75 — 100 | 50 — 75 | 100 | 100 | 37 | 65 |
| BssEC I | GGNNC↓C |
Y | — | 50 — 75 | 50 — 75 | 50 — 75 | 75 — 100 | 100 | 100 | 60 | 80 |
| BssNA I | CAT↓ATG |
W | + | 50 — 75 | 50 — 75 | 75 — 100 | 100 | 75 — 100 | 100 | 37 | N |
| BssT1 I | GGWWC↓C |
2K | — | 10 — 25 | 25 — 50 | 25 — 50 | 75 — 100 | 10 — 25 | 100 | 60 | 80 |
| Bst2B I | GAGCA↓C |
Y | + | 75 — 100 | 25 — 50 | 10 — 25 | 25 — 50 | 100 | 100 | 60 | 80 |
| Bst2U I | GGW↓CC |
G | + | 75 — 100 | 100 | 50 — 75 | 50 — 75 | 10 — 25 | 50 | 60 | N |
| Bst4C I | TG↓NCA |
Y | — | 75 — 100 | 75 — 100 | 10 — 25 | 25 — 50 | 100 | 50 | 65 | N |
| Bst6 I | GAGAAG(N)4↓ |
Y | + | 75 — 100 | 75 — 100 | 50 — 75 | 75 — 100 | 100 | 100 | 65 | 80 |
| BstAC I | CYGC↓RG |
W | — | 75 — 100 | 75 — 100 | 50 — 75 | 100 | 75 — 100 | 100 | 37 | 80 |
| BstAF I | GAATT↓C |
W | + | 10 — 25 | 25 — 50 | 75 — 100 | 100 | 25 — 50 | 100 | 55 | 80 |
| BstAP I | CGTN↓NNNNACG |
W | — | 25 — 50 | 25 — 50 | 75 — 100 | 100 | 25 — 50 | 100 | 60 | 80 |
| BstAU I | ACATG↓T |
W | — | 10 — 25 | 50 — 75 | 25 — 50 | 100 | 25 — 50 | 100 | 37 | 80 |
| BstBA I | RTG↓CAY |
W | + | 25 — 50 | 25 — 50 | 75 — 100 | 100 | 25 — 50 | 50 | 65 | 80 |
| BstC8I | CGN↓NCG |
Y | — | 10 — 25 | 25 — 50 | 50 — 75 | 75 — 100 | 100 | 100 | 55 | 80 |
| BstDE I | GANT↓C |
G | — | 75 — 100 | 100 | 25 — 50 | 50 — 75 | 10 — 25 | 100 | 60 | 80 |
| BstDS I | GGYRC↓C |
Y | — | 0 — 10 | 75 — 100 | 50 — 75 | 25 — 50 | 100 | 100 | 65 | 80 |
| BstEN I | GGANNN↓NNTCC |
Y | — | 50 — 75 | 50 — 75 | 25 — 50 | 25 — 50 | 100 | 100 | 65 | 80 |
| BstF5 I | CCTAC↓NN |
Y | — | 75 — 100 | 50 — 75 | 25 — 50 | 50 — 75 | 100 | 100 | 65 | 80 |
| BstFN I | GC↓GC |
Y | — | 75 — 100 | 50 — 75 | 25 — 50 | 25 — 50 | 100 | 75 | 60 | 80 |
| BstH2 I | Y↓CGCGR |
Y | + | 50 — 75 | 50 — 75 | 0 — 10 | 10 — 25 | 100 | 100 | 65 | 80 |
| BstHH I | C↓GCG |
Y | + | 75 — 100 | 50 — 75 | 25 — 50 | 50 — 75 | 100 | 100 | 50 | N |
| BstKTI | C↓TAG |
W | — | 25 — 50 | 50 — 75 | 75 — 100 | 100 | 50 — 75 | 100 | 37 | 65 |
| BstMA I | CAGAG(N)5↓ |
W | + | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 75 — 100 | 100 | 55 | 65 |
| BstMB I | CTAG↓ |
O | — | 10 — 25 | 25 — 50 | 100 | 75 — 100 | 10 — 25 | 100 | 65 | 80 |
| BstMC I | GC↓YRGC |
B | + | 100 | 75 — 100 | 10 — 25 | 10 — 25 | 50 — 75 | 100 | 50 | 80 |
| BstMW I | CGNN↓NNNNNCG |
Y | — | 10 — 25 | 25 — 50 | 25 — 50 | 50 — 75 | 100 | 40 | 55 | 80 |
| BstNS I | Y↓GTACR |
B | + | 100 | 50 — 75 | 10 — 25 | 10 — 25 | 75 — 100 | 50 | 37 | 65 |
| BstPA I | CTGNN↓NNCAG |
Y | — | 50 — 75 | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 100 | 65 | N |
| BstSCI | GGNCC↓ |
Y | — | 50 — 75 | 50 — 75 | 50 — 75 | 50 — 75 | 100 | 60 | 55 | 80 |
| BstSF I | GAYRT↓C |
O | + | 75 — 100 | 25 — 50 | 100 | 50 — 75 | 50 — 75 | 100 | 60 | N |
| BstSL I | C↓MCGKG |
G | + | 50 — 75 | 100 | 50 — 75 | 75 — 100 | 75 — 100 | 100 | 55 | 65 |
| BstSN I | ATG↓CAT |
B | — | 100 | 50 — 75 | 0 — 10 | 10 — 25 | 50 — 75 | 50 | 37 | 80 |
| BstV1I | CGTCG(N)12↓ |
G | — | 75 — 100 | 100 | 75 — 100 | 75 — 100 | 75 — 100 | 100 | 55 | 80 |
| BstV2 I | CTTCTG(N)6↓ |
Y | + | 75 — 100 | 75 — 100 | 25 — 50 | 25 — 50 | 100 | 70 | 55 | 65 |
| BstX I | GGTN↓NNNNNACC |
O | — | 10 — 25 | 10 — 25 | 100 | 75 — 100 | 25 — 50 | 100 | 37 | 65 |
| BstX2 I Temporarily unavailable |
YCTAG↓R |
G | — | 75 — 100 | 100 | 0 — 10 | 10 — 25 | 25 — 50 | 100 | 60 | 80 |
| Bsu I | CATAGG(N)5↓ |
Y | — | 75 — 100 | 50 — 75 | 10 — 25 | 25 — 50 | 100 | 10 | 37 | 65 |
| BsuR I | CC↓GG |
G | — | 75 — 100 | 100 | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 37 | 80 |
| Btr I | GTG↓CAG |
O | + | 75 — 100 | 75 — 100 | 100 | 75 — 100 | 75 — 100 | 100 | 60 | 80 |
| Cci I | AGTAC↓T |
W | + | 0 — 10 | 10 — 25 | 25 — 50 | 100 | 75 — 100 | 100 | 55 | 80 |
| CciN I | CGCCGG↓CG |
Y | — | 25 — 50 | 50 — 75 | 75 — 100 | 75 — 100 | 100 | 100 | 37 | 65 |
| Dra I | AAA↓TTT |
G | + | 75 — 100 | 100 | 25 — 50 | 75 — 100 | 75 — 100 | 100 | 37 | 65 |
| DraIII | GTG↓NNNCAC |
2K | + | 25 — 50 | 50 — 75 | 75 — 100 | 75 — 100 | 50 — 75 | 100 | 37 | 65 |
| Dri I | CTGNN↓NNNCAG |
Y | — | 75 — 100 | 75 — 100 | 10 — 25 | 10 — 25 | 100 | 40 | 37 | 65 |
| DseD I | CTGNN↓NNNNCAG |
Y | + | 75 — 100 | 75 — 100 | 25 — 50 | 50 — 75 | 100 | 30 | 37 | 80 |
| EcoICR I | CTC↓GAG |
G | + | 75 — 100 | 100 | 0 — 10 | 0 — 10 | 75 — 100 | 5 | 37 | 65 |
| EcoR I | CTTAA↓G |
* | + | 50 — 75 | 75 — 100 | 75 — 100 | 75 — 100 | 50 — 75 | 50 | 37 | 65 |
| EcoR V | CTA↓TAG |
W | + | 0 — 10 | 25 — 50 | 50 — 75 | 100 | 25 — 50 | 50 | 37 | 80 |
| Ege I | CCG↓CGG |
B | + | 100 | 75 — 100 | 10 — 25 | 50 — 75 | 75 — 100 | 100 | 37 | 65 |
| Erh I | GGWWC↓C |
2W | + | 10 — 25 | 25 — 50 | 50 — 75 | 75 — 100 | 10 — 25 | 100 | 37 | 65 |
| FaeI | ↓GTAC |
* | + | 25 — 50 | 50 — 75 | 10 — 25 | 10 — 25 | 75 — 100 | 100 | 37 | 65 |
| FaiI | RT↓AY |
B | — | 100 | 50 — 75 | 10 — 25 | 25 — 50 | 25 — 50 | 100 | 50 | 80 |
| FalI | ↓(N)13TTCNNNNNGAA(N)8↓ |
W+ SAM | — | 0 — 10 | 25 — 50 | 75 — 100 | 100 | 50 — 75 | 70 | 37 | 65 |
| FatI | GTAC↓ |
G | — | 10 — 25 | 100 | 25 — 50 | 10 — 25 | 50 — 75 | 100 | 55 | 65 |
| Fau I | GGGCG(N)6↓ |
B | — | 100 | 25 — 50 | 0 — 10 | 0 — 10 | 50 — 75 | 50 | 55 | 65 |
| FauND I | GTAT↓AC |
Y | + | 50 — 75 | 75 — 100 | 10 — 25 | 50 — 75 | 100 | 100 | 37 | 65 |
| Fbl I | CAKM↓TG |
Y | — | 50 — 75 | 75 — 100 | 0 — 10 | 50 — 75 | 100 | 100 | 55 | 80 |
| Fok I | CCTAC(N)13↓ |
Y | — | 50 — 75 | 50 — 75 | 25 — 50 | 25 — 50 | 100 | 100 | 37 | 65 |
| FriO I | C↓YCGRG |
Y | + | 75 — 100 | 75 — 100 | 10 — 25 | 0 — 10 | 100 | 25 | 37 | 65 |
| Fsp4H I | CGN↓CG |
Y | — | 50 — 75 | 75 — 100 | 10 — 25 | 25 — 50 | 100 | 100 | 37 | 65 |
| Gsa I | G↓GGTCG |
W | + | 10 — 25 | 25 — 50 | 75 — 100 | 100 | 75 — 100 | 100 | 70 | N |
| Hae III | CC↓GG |
G | — | 75 — 100 | 100 | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 37 | 80 |
| Hga I | CTGCG(N)10↓ |
B | — | 100 | 75 — 100 | 10 — 25 | 25 — 50 | 50 — 75 | 50 | 37 | 65 |
| Hind II | CAR↓YTG |
G | + | 75 — 100 | 100 | 25 — 50 | 25 — 50 | 75 — 100 | 50 | 37 | 65 |
| Hind III | TTCGA↓A |
W | + | 10 — 25 | 25 — 50 | 0 — 10 | 100 | 0 — 10 | 100 | 37 | 80 |
| Hinf I | CTNA↓G |
O | — | 25 — 50 | 75 — 100 | 100 | 75 — 100 | 75 — 100 | 100 | 37 | 80 |
| Hpa I | CAA↓TTG |
Y | — | 0 — 10 | 50 — 75 | 10 — 25 | 25 — 50 | 100 | 25 | 37 | 65 |
| Hpa II | GGC↓C |
B | — | 100 | 50 — 75 | 10 — 25 | 25 — 50 | 50 — 75 | 50 | 37 | 80 |
| HpySE526 I | TGC↓A |
Y | — | 75 — 100 | 75 — 100 | 10 — 25 | 25 — 50 | 100 | 50 | 37 | 65 |
| HspA I | CGC↓G |
Y | — | 50 — 75 | 50 — 75 | 25 — 50 | 25 — 50 | 100 | 100 | 37 | 80 |
| Kpn I | C↓CATGG |
B | + | 100 | 25 — 50 | 25 — 50 | 25 — 50 | 75 — 100 | 50 | 37 | 80 |
| Ksp22 I | ACTAG↓T |
2K | + | 50 — 75 | 75 — 100 | 50 — 75 | 50 — 75 | 25 — 50 | 100 | 37 | 65 |
| Kzo9 I | CTAG↓ |
G | — | 50 — 75 | 100 | 50 — 75 | 50 — 75 | 50 — 75 | 100 | 37 | 65 |
| Lmn I | CGAG↓GN |
B | — | 100 | 75 — 100 | 50 — 75 | 50 — 75 | 75 — 100 | 80 | 37 | 65 |
| Mab I | TGGWCC↓A |
W | + | 25 — 50 | 50 — 75 | 75 — 100 | 100 | 50 — 75 | 100 | 37 | 65 |
| Mbo II | CTTCT(N)7↓ |
Y | — | 75 — 100 | 75 — 100 | 25 — 50 | 50 — 75 | 100 | 50 | 37 | 65 |
| Mfe I | GTTAA↓C |
B | + | 100 | 75 — 100 | 10 — 25 | 25 — 50 | 75 — 100 | 25 | 37 | N |
| Mhl I | C↓HCGDG |
W | — | 10 — 25 | 25 — 50 | 75 — 100 | 100 | 10 — 25 | 100 | 37 | 80 |
| Mlu I | TGCGC↓A |
O | — | 0 — 10 | 10 — 25 | 100 | 25 — 50 | 10 — 25 | 50 | 37 | 65 |
| Mly113 I | CCGC↓GG |
B | — | 100 | 25 — 50 | 10 — 25 | 10 — 25 | 50 — 75 | 100 | 37 | 65 |
| Mnl I | GGAG(N)6↓ |
G | + | 75 — 100 | 100 | 25 — 50 | 25 — 50 | 75 — 100 | 100 | 37 | 65 |
| Mox20 I | ACC↓GGT |
O | — | 10 — 25 | 25 — 50 | 100 | 75 — 100 | 25 — 50 | 75 | 37 | N |
| MroN I | CGGCC↓G |
B | — | 100 | 50 — 75 | 10 — 25 | 0 — 10 | 10 — 25 | 5 | 37 | 80 |
| MroX I | CTTNN↓NNAAG |
W | — | 50 — 75 | 50 — 75 | 50 — 75 | 100 | 25 — 50 | 100 | 37 | 65 |
| Msp I | GGC↓C |
B | — | 100 | 75 — 100 | 50 — 75 | 75 — 100 | 75 — 100 | 100 | 37 | 65 |
| MspA1 I | GKC↓GMC |
Y | + | 10 — 25 | 75 — 100 | 10 — 25 | 25 — 50 | 100 | 100 | 37 | 65 |
| MspR9 I | GGN↓CC |
O | — | 50 — 75 | 50 — 75 | 100 | 50 — 75 | 50 — 75 | 100 | 37 | 80 |
| Nru I | AGC↓GCT |
W | — | 0 — 10 | 10 — 25 | 75 — 100 | 100 | 10 — 25 | 100 | 37 | 80 |
| PalA I | CCGCGC↓GG |
Y | — | 25 — 50 | 10 — 25 | 75 — 100 | 100 | 100 | 40 | 37 | 65 |
| Pec I | Y | — | 50-75 | 75 — 100 | 10 — 25 | 25 — 50 | 100 | 100 | 37 | 65 | |
| Pce I | TCC↓GGA |
Y | — | 75 — 100 | 75 — 100 | 50 — 75 | 25 — 50 | 100 | 100 | 50 | 80 |
| Pci I | TGTAC↓A |
O | — | 50 — 75 | 75 — 100 | 100 | 75 — 100 | 50 — 75 | 50 | 37 | 65 |
| PciS I | CGAGAAG(N)4↓ |
B | — | 100 | 50 — 75 | 0 — 10 | 0 — 10 | 75 — 100 | 50 | 37 | 65 |
| Pct I | CTTAC↓GN |
O | — | 25 — 50 | 50 — 75 | 100 | 75 — 100 | 10 — 25 | 50 | 37 | 65 |
| Ple19 I | GC↓TAGC |
Y | — | 75 — 100 | 75 — 100 | 25 — 50 | 25 — 50 | 100 | 100 | 37 | 65 |
| Pps I | CTCAG(N)5↓ |
Y | + | 50 — 75 | 10 — 25 | 0 — 10 | 25 — 50 | 100 | 50 | 37 | 65 |
| Pse31 I | CCAGAG(N)5↓ |
Y | — | 25-50 | 75 — 100 | 10 — 25 | 25 — 50 | 100 | 75 | 37 | 65 |
| Psi I | AAT↓ATT |
B | — | 100 | 25 — 50 | 10 — 25 | 25 — 50 | 75 — 100 | 40 | 37 | 65 |
| Psp124B I | C↓TCGAG |
G | — | 75 — 100 | 100 | 10 — 25 | 0 — 10 | 75 — 100 | 30 | 37 | 80 |
| Psp6 I | GGWCC↓ |
B | — | 100 | 50 — 75 | 10 — 25 | 25 — 50 | 75 — 100 | 10 | 55 | 80 |
| PspC I | GTG↓CAC |
B | + | 100 | 50 — 75 | 0 — 10 | 0 — 10 | 50 — 75 | 5 | 37 | 65 |
| PspE I | CCANTG↓G |
B | — | 100 | 50 — 75 | 25 — 50 | 50 — 75 | 50 — 75 | 100 | 37 | 65 |
| PspL I | GCATG↓C |
Y | + | 75 — 100 | 75 — 100 | 25 — 50 | 10 — 25 | 100 | 100 | 37 | 65 |
| PspN4 I | CCN↓NGG |
Y | — | 10 — 25 | 10 — 25 | 10 — 25 | 25 — 50 | 100 | 50 | 37 | 65 |
| PspOM I | CCCGG↓G |
Y | — | 75 — 100 | 10 — 25 | 0 — 10 | 0 — 10 | 100 | 25 | 37 | 65 |
| PspPP I | YCCWG↓GR |
Y | — | 10 — 25 | 10 — 25 | 0 — 10 | 0 — 10 | 100 | 100 | 37 | 65 |
| PspX I | BGAGCT↓CV |
Y | + | 50 — 75 | 50 — 75 | 25 — 50 | 75 — 100 | 100 | 25 | 37 | 80 |
| Psr I | ↓(N)12CTTGNNNNNNATG(N)7↓ |
Y | + | 10 — 25 | 10 — 25 | 0 — 10 | 0 — 10 | 100 | 30 | 30 | 65 |
| Pst I | G↓ACGTC |
O | + | 10 — 25 | 25 — 50 | 100 | 25 — 50 | 25 — 50 | 50 | 37 | 80 |
| PstN I | GTC↓NNNGAC |
Y | — | 25 — 50 | 50 — 75 | 10 — 25 | 25 — 50 | 100 | 100 | 37 | 65 |
| Pvu II | GTC↓GAC |
G | + | 25 — 50 | 100 | 25 — 50 | 25 — 50 | 25 — 50 | 100 | 37 | 80 |
| Rga I | CGC↓TAGCG |
Y | — | 75 — 100 | 50 — 75 | 10 — 25 | 25 — 50 | 100 | 100 | 55 | 80 |
| Rig I | CC↓GGCCGG |
* | + | 75 — 100 | 50 — 75 | 0 — 10 | 10 — 25 | 50 — 75 | 10 | 37 | 65 |
| Rsa I | CA↓TG |
B | — | 100 | 50 — 75 | 0 — 10 | 50 — 75 | 75 — 100 | 50 | 37 | N |
| RsaN I | CAT↓G |
B | — | 100 | 75 — 100 | 50 — 75 | 50 — 75 | 75 — 100 | 100 | 37 | 80 |
| Rsr2 I | GCCWG↓GC |
Y | + | 50 — 75 | 75 — 100 | 0 — 10 | 10 — 25 | 100 | 25 | 37 | 65 |
| Sal I | CAGCT↓G |
O | — | 0 — 10 | 10 — 25 | 100 | 25 — 50 | 0 — 10 | 5 | 37 | 65 |
| Sbf I | GG↓ACGTCC |
Y | — | 75 — 100 | 50 — 75 | 0 — 10 | 0 — 10 | 100 | 50 | 37 | 80 |
| Set I | ↓TSSA |
Y | — | 25 — 50 | 25 — 50 | 75 — 100 | 75 — 100 | 100 | 100 | 55 | 80 |
| SfaN I | CGTAG(N)9↓ |
O | — | 10 — 25 | 25 — 50 | 100 | 75 — 100 | 0 — 10 | 25 | 37 | 80 |
| Sfi I | CCGGN↓NNNNCCGG |
G | + | 75 — 100 | 100 | 25 — 50 | 25 — 50 | 25 — 50 | 75 | 50 | 65 |
| Sfr274 I | GAGCT↓C |
B | — | 100 | 75 — 100 | 50 — 75 | 50 — 75 | 75 — 100 | 100 | 50 | 65 |
| Sfr303 I | GG↓CGCC |
B | — | 100 | 50 — 75 | 10 — 25 | 10 — 25 | 75 — 100 | 100 | 37 | 65 |
| Sma I | GGG↓CCC |
Y | — | 0 — 10 | 0 — 10 | 0 — 10 | 0 — 10 | 100 | 50 | 25 | 65 |
| Smi I | TAAA↓TTTA |
O | + | 25 — 50 | 25 — 50 | 100 | 75 — 100 | 25 — 50 | 10 | 37 | 65 |
| SmiM I | GTRNN↓NNYAC |
W | — | 10 — 25 | 10 — 25 | 75 — 100 | 100 | 10 — 25 | 60 | 37 | 65 |
| Sph I | C↓GTACG |
G | + | 25 — 50 | 100 | 75 — 100 | 75 — 100 | 50 — 75 | 100 | 37 | 65 |
| Sse9 I | TTAA↓ |
B | + | 100 | 75 — 100 | 50 — 75 | 50 — 75 | 75 — 100 | 75 | 55 | 65 |
| Ssp I | TTA↓TAA |
K | + | 75 — 100 | 50 — 75 | 25 — 50 | 50 — 75 | 75 — 100 | 100 | 37 | 65 |
| Taq I | AGC↓T |
Y | + | 50 — 75 | 75 — 100 | 75 — 100 | 50 — 75 | 100 | 100 | 65 | 80 |
| Tru9 I | AAT↓T |
W | — | 75 — 100 | 25 — 50 | 25 — 50 | 100 | 50 — 75 | 100 | 65 | 80 |
| TseF I | CASTG↓ |
B | — | 100 | 50 — 75 | 0 — 10 | 25 — 50 | 50 — 75 | 50 | 65 | N |
| Tth111 I | CTGNN↓NCAG |
Y | — | 75 — 100 | 50 — 75 | 10 — 25 | 10 — 25 | 100 | 100 | 65 | 80 |
| Vne I | CACGT↓G |
O | — | 10 — 25 | 25 — 50 | 100 | 25 — 50 | 25 — 50 | 100 | 37 | 65 |
| Vsp I | TAAT↓TA |
W | — | 0 — 10 | 10 — 25 | 50 — 75 | 100 | 25 — 50 | 50 | 37 | 65 |
| Xba I | AGATC↓T |
O | + | 75 — 100 | 75 — 100 | 100 | 50 — 75 | 75 — 100 | 25 | 37 | 65 |
| Xma I | GGGCC↓C |
Y | — | 75 — 100 | 50 — 75 | 0 — 10 | 0 — 10 | 100 | 50 | 37 | 65 |
| ZraI | CTG↓CAG |
B | — | 100 | 50 — 75 | 25 — 50 | 25 — 50 | 75 — 100 | 100 | 37 | 80 |
| Zrm I | TCA↓TGA |
Y | + | 50 — 75 | 25 — 50 | 0 — 10 | 0 — 10 | 100 | 50 | 37 | 65 |
| Zsp2 I | T↓ACGTA |
B | + | 100 | 50 — 75 | 25 — 50 | 25 — 50 | 25 — 50 | 50 | 37 | 65 |
B
G
O
W
Y
Rose
G
O
W
Y
Rose