Блокирование активности рестриктаз при полном или частичном перекрывании их сайтов узнавания метилированием
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Рестриктаза |
Позиция гидролиза |
Прототип |
Блокирование |
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сопряжённой ДНК |
dam |
dcm |
M.SssI |
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AatII |
GACGT↑C |
AatII |
G(m6A)CGTC |
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AbsI |
CC↑TCGAGG |
AbsI |
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Acc16I |
TGC↑GCA |
MstI |
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Acc36I |
ACCTGC(N)4↑ |
BspMI |
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Acc65I |
G↑GTACC |
KpnI |
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+ |
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AccB1I |
G↑GYRCC |
HgiCI |
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AccB7I |
CCANNNN↑NTGG |
PflMI |
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+ |
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AccBSI |
GAG↑CGG |
BsrBI |
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AclI |
AA↑CGTT |
AclI |
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+ |
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AclWI |
GGATC(N)4↑ |
BinI |
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+ |
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AcoI |
Y↑GGCCR |
CfrI |
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+ |
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AcsI |
R↑AATTY |
ApoI |
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AcuI |
CTGAAG(N)16↑ |
Eco57I |
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AfeI |
AGC↑GCT |
Eco47III |
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AgsI |
TTS↑AA |
AgsI |
TTSA(m6A) |
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AhlI |
A↑CTAGT |
SpeI |
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AjnI |
↑CCWGG |
EcoRII |
(m5C)CWGG |
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– |
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AluI |
AG↑CT |
AluI |
AG(m5C)T |
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AluBI |
AG↑CT |
AluI |
(m6A)GCT |
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Ama87I |
C↑YCGRG |
AvaI |
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AoxI |
↑RG(5mC)Y |
AoxI |
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ApaI |
GGGCC↑C |
ApaI |
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+ |
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ArsI |
↑(N)8GACNNNNNNTTYG(N)11↑ |
ArsI |
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AsiGI |
A↑CCGGT |
AgeI |
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AsiSI |
GCGAT↑CGC |
SgfI |
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– |
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+ |
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AspA2I |
C↑CTAGG |
AvrII |
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AspLEI |
GCG↑C |
HhaI |
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+ |
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AspS9I |
G↑GNCC |
Sau96I |
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+ |
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AsuC2I |
CC↑SGG |
CauII |
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AsuHPI |
GGTGA(N)8↑ |
HphI |
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+ |
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AsuNHI |
G↑CTAGC |
NheI |
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BamHI |
G↑GATCC |
BamHI |
GGAT(m4C)C |
– |
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BarI |
↑(N)7GAAGNNNNNNTAC(N)12↑ |
BarI |
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Bbv12I |
GWGCW↑C |
HgiAI |
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BglI |
GCCNNNN↑NGGC |
BglI |
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– |
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BglII |
A↑GATCT |
BglII |
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– |
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BlsI |
Последовательность |
BlsI |
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Bme18I |
G↑GWCC |
AvaII |
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+ |
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BmtI |
GCTAG↑C |
NheI |
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BmuI |
ACTGGG(N)5↑ |
BfiI |
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BpmI |
CTGGAG(N)16↑ |
GsuI |
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Bpu10I |
CC↑TNAGC |
Bpu10I |
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Bpu14I |
TT↑CGAA |
AsuII |
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Bsa29I |
AT↑CGAT |
ClaI |
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+ |
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Bsc4I |
CCNNNNN↑NNGG |
BsiYI |
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Bse1I |
ACTGGN↑ |
BsrI |
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Bse118I |
R↑CCGGY |
Cfr10I |
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Bse21I |
CC↑TNAGG |
SauI |
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Bse3DI |
GCAATGNN↑ |
BsrDI |
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Bse8I |
GATNN↑NNATC |
BsaBI |
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BsePI |
G↑CGCGC |
BsePI |
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BseX3I |
C↑GGCCG |
XmaIII |
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BslFI |
GGGAC(N)10↑ |
FinI |
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Bso31I |
GGTCTCN↑ |
Eco31I |
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Bsp13I |
T↑CCGGA |
BspMII |
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+ |
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Bsp1720I |
GC↑TNAGC |
EspI |
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Bsp19I |
C↑CATGG |
NcoI |
(m4C)CATGG |
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BspACI |
C↑CGC |
AciI |
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+ |
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BspFNI |
CG↑CG |
FnuDII |
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+ |
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BssECI |
C↑CNNGG |
SecI |
(m4C)CNNGG |
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BssNAI |
GTA↑TAC |
SnaI |
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BssT1I |
C↑CWWGG |
StyI |
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Bst2BI |
C↑TCGTG |
BsiI |
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Bst2UI |
CC↑WGG |
BstNI |
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– |
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Bst4CI |
ACN↑GT |
Tsp4CI |
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Bst6I |
CTCTTCN↑ |
Ksp632I |
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BstACI |
GR↑CGYC |
AcyI |
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BstAFI |
C↑TTAAG |
AflII |
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BstAPI |
GCANNNN↑NTGC |
(ApaBI) |
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BstAUI |
T↑GTACA |
Bsp1407I |
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BstBAI |
YAC↑GTR |
BsaAI |
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+ |
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BstC8I |
GCN↑NGC |
Cac8I |
G(m5C)NNGC |
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BstDEI |
C↑TNAG |
DdeI |
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BstDSI |
C↑CRYGG |
DsaI |
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BstENI |
CCTNN↑NNNAGG |
EcoNI |
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– |
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BstF5I |
GGATGNN↑ |
(FokI) |
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BstFNI |
CG↑CG |
FnuDII |
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+ |
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BstH2I |
RGCGC↑Y |
HaeII |
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BstHHI |
GCG↑C |
HhaI |
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+ |
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BstKTI |
GAT↑C |
MboI |
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+ |
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BstMAI |
GTCTCN↑ |
BsmAI |
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BstMBI |
↑GATC |
MboI |
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+ |
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BstMCI |
CGRY↑CG |
McrI |
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BstMWI |
GCNNNNN↑NNGC |
MwoI |
G(m5C)NNNNNNNGC |
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BstNSI |
RCATG↑Y |
NspI |
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BstPAI |
GACNN↑NNGTC |
PshAI |
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BstSCI |
↑CCNGG |
ScrFI |
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+ |
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BstSFI |
C↑TRYAG |
SfeI |
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BstSLI |
GKGCM↑C |
BseSI |
GKG(m5C)MC |
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– |
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BstSNI |
TAC↑GTA |
SnaBI |
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BstV1I |
GCAGC(N)8↑ |
BbvI |
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BstV2I |
GAAGAC(N)2↑ |
BbvII |
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BstXI |
CCANNNNN↑NTGG |
BstXI |
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BstX2I |
R↑GATCY |
XhoII |
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– |
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BstX2I |
R↑GATCY |
XhoII |
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BsuI |
GTATCC(N)6↑ |
BciVI |
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BsuRI |
GG↑CC |
HaeIII |
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BtrI |
CAC↑GTC |
BtrI |
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CciI |
T↑CATGA |
BspHI |
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CciNI |
GC↑GGCCGC |
NotI |
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+ |
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DraI |
TTT↑AAA |
AhaIII |
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DraIII |
CACNNN↑GTG |
DraIII |
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DriI |
GACNNN↑NNGTC |
Eam1105I |
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DseDI |
GACNNNN↑NNGTC |
DrdI |
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EcoICRI |
GAG↑CTC |
SacI |
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EcoRI |
G↑AATTC |
EcoRI |
GA(m6A)TTC |
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EcoRV |
GAT↑ATC |
EcoRV |
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EgeI |
GGC↑GCC |
NarI |
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ErhI |
C↑CWWGG |
StyI |
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FaeI |
CATG↑ |
NlaIII |
C(m6A)TG |
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FaiI |
YA↑TR |
FaiI |
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FalI |
↑(N)8AAGNNNNNCTT(N)13↑ |
FalI |
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FatI |
↑CATG |
NlaIII |
(m5C)ATG |
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FauI |
CCCGC(N)4↑ |
FauI |
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+ |
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FauNDI |
CA↑TATG |
NdeI |
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FblI |
GT↑MKAC |
AccI |
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FokI |
GGATG(N)9↑ |
FokI |
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FriOI |
GRGCY↑C |
BanII |
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Fsp4HI |
GC↑NGC |
Fnu4HI |
G(m5C)NGC |
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GlaI |
R(5mC)↑GY |
GlaI |
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GluI |
G(5mC)↑NG(5mC) |
GluI |
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GsaI |
CCCAG↑C |
BseYI |
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HaeIII |
GG↑CC |
HaeIII |
GG(m5C)C |
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HgaI |
GACGC(N)5↑ |
HgaI |
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+ |
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HindII |
GTY↑RAC |
HindII |
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HindIII |
A↑AGCTT |
HindIII |
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HinfI |
G↑ANTC |
HinfI |
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HpaI |
GTT↑AAC |
HpaI |
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+ |
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HpaII |
C↑CGG |
HpaII |
C(m5C)GG, а так же |
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+ |
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HpySE526I |
A↑CGT |
MaeII |
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HspAI |
G↑CGC |
HhaI |
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+ |
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KpnI |
GGTAC↑C |
KpnI |
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– |
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KroI |
G↑C(5mC)GGC |
KroI |
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KroNI |
GCC↑GGC |
NaeI |
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+ |
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Ksp22I |
T↑GATCA |
BclI |
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+ |
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Kzo9I |
↑GATC |
MboI |
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– |
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+ |
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LmnI |
GCTCCN↑ |
LmnI |
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MabI |
A↑CCWGGT |
SexAI |
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– |
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MalI |
G(mA)↑TC |
DpnI |
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MboII |
GAAGA(N)8↑ |
MboII |
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+ |
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MfeI |
C↑AATTG |
MfeI |
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MhlI |
GDGCH↑C |
SduI |
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MluI |
A↑CGCGT |
MluI |
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Mly113I |
GG↑CGCC |
NarI |
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MnlI |
CCTC(N)7↑ |
MnlI |
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+ |
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Mox20I |
TGG↑CCA |
BalI |
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+ |
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MroNI |
G↑CCGGC |
(NaeI) |
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MroXI |
GAANN↑NNTTC |
XmnI |
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MspI |
C↑CGG |
HpaII |
(m5C)CGG |
|
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– |
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MspA1I |
CMG↑CKG |
NspBII |
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MspR9I |
CC↑NGG |
ScrFI |
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+ |
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MteI |
G(5mC)G(5mC)↑NG(5mC)G(5mC) |
Mte |
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NruI |
TCG↑CGA |
NruI |
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+ |
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PalAI |
GG↑CGCGCC |
AscI |
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+ |
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PceI |
AGG↑CCT |
StuI |
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PciI |
A↑CATGT |
BspLU11I |
(m6A)CATGT |
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PciSI |
GCTCTTCN↑ |
SapI |
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PcsI |
(5mC)GNNNNN↑NN(5mC)G |
PcsI |
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PctI |
GAATGCN↑ |
BsmI |
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PkrI |
Последовательность |
PkrI |
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Ple19I |
CGAT↑CG |
PvuI |
|
– |
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PpsI |
GAGTC(N)4↑ |
PleI |
|
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PsiI |
TTA↑TAA |
PsiI |
|
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Psp124BI |
GAGCT↑C |
SacI |
|
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Psp6I |
↑CCWGG |
EcoRII |
|
+ |
|
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PspCI |
CAC↑GTG |
PmaCI |
|
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PspEI |
G↑GTNACC |
BstEII |
|
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PspLI |
C↑GTACG |
SplI |
|
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PspN4I |
GGN↑NCC |
NlaIV |
GGNN(m5C)C |
|
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PspOMI |
G↑GGCCC |
(ApaI) |
|
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PspPPI |
RG↑GWCCY |
PpuMI |
|
|
+ |
|
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PspXI |
VC↑TCGAGB |
PspXI |
|
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|
|
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PsrI |
↑(N)7GAACNNNNNNTAC(N)12↑ |
PsrI |
|
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PstI |
CTGCA↑G |
PstI |
|
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PstNI |
CAGNNN↑CTG |
AlwNI |
|
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PvuII |
CAG↑CTG |
PvuII |
|
|
|
|
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RgaI |
GCGAT↑CGC |
SgfI |
|
– |
|
|
|
RigI |
GGCCGG↑CC |
FseI |
GG(m5C)CGGCC |
|
|
+ |
|
RsaI |
GT↑AC |
RsaI |
|
|
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|
|
RsaNI |
G↑TAC |
RsaI |
|
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Rsr2I |
CG↑GWCCG |
RsrII |
|
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SalI |
G↑TCGAC |
SalI |
|
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SbfI |
CCTGCA↑GG |
Sse8387I |
|
|
|
|
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SetI |
ASST↑ |
SetI |
|
|
|
|
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SfaNI |
GCATC(N)5↑ |
SfaNI |
|
|
|
|
|
SfiI |
GGCCNNNN↑NGGCC |
SfiI |
|
|
+ |
|
|
Sfr274I |
C↑TCGAG |
XhoI |
CTCG(m6A)G |
|
|
|
|
Sfr303I |
CCGC↑GG |
SacII |
|
|
|
|
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SmaI |
CCC↑GGG |
SmaI |
|
|
|
|
|
SmiI |
ATTT↑AAAT |
SwaI |
|
|
|
|
|
SmiMI |
CAYNN↑NNRTG |
MslI |
|
|
|
|
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SphI |
GCATG↑C |
SphI |
|
|
|
|
|
Sse9I |
↑AATT |
Tsp509I |
A(m6A)TT |
|
|
|
|
SspI |
AAT↑ATT |
SspI |
A(m6A)TATT |
|
|
|
|
TaqI |
T↑CGA |
TaqI |
|
|
|
|
|
Tru9I |
T↑TAA |
MseI |
TTA(m6A) |
|
|
|
|
TseFI |
↑GTSAC |
Tsp45I |
|
|
|
|
|
Tth111I |
GACN↑NNGTC |
Tth111I |
|
|
|
|
|
VneI |
G↑TGCAC |
ApaLI |
|
|
|
|
|
VspI |
AT↑TAAT |
VspI |
ATTA(m6A)T |
|
|
|
|
XbaI |
T↑CTAGA |
XbaI |
|
+ |
|
|
|
XmaI |
C↑CCGGG |
XmaI |
|
|
|
|
|
ZraI |
GAC↑GTC |
(AatII) |
|
|
|
|
|
ZrmI |
AGT↑ACT |
ScaI |
|
|
|
|
|
Zsp2I |
ATGCA↑T |
NsiI |
|
|
|
|