PstN I

PstN I

Price range: 6 500 ₽ through 26 000 ₽

Restriction endonuclease PstN I

  • Recognition site:
    5'… CAGNNN▼CTG …3'
    3'… GTC▲NNNGAC …5'
  • Source: Pseudomonas stutzeri 217
  • Optimal buffer: SE-buffer Y
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E571 Category:

SKUРackagePackageVariantConcentrationPrice 
E571S500 uRegular5 000-10 000 u/ml6 500 ₽
E572L2500 uRegular5 000-10 000 u/ml26 000 ₽

Description

Recognition site and hydrolysis position:

CAGNNNCTG CAGNNN↑CTG
GTCNNNGAC GTC↓NNNGAC

Source: Pseudomonas stutzeri 217
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
25 50 10 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme > 95% of Lambda DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: When using a buffer other than the optimal (suppied) SEBuffer, it may benecessary to add more enzyme to achieve complete digestion.
References:

Additional Information

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