Нуклеотидная последовательность плазмиды pHspAI2
pHspAI2 (4118 п.о.) является модификацией плазмиды pHspAI1. Помимо фрагмента геномной ДНК бактериального штамма Haemophilus species A1, включающего ген ДНК-метилтрансферазы M.HspAI, pHspAI2 содержит единственный канонический сайт узнавания GlaI.
> TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CTCTTTCCAA TTTTCAATCT ATCTGCAATA TCTTTTTCGT TTTTAAAACC ATCTTTAGCT GTTTTAGATC CTAATTCTAC CAGATTCATT TTATTTACCT TTATTTATAG AGATTAGTAA TTTTTCTGCG ATTTTCTGTA TTACATTAAC CGTCATCGCA TTTCCAGCTT GGGATAAAAT CTTACTATCG GGTAATTTAT AATCAATGGC TTTTTGAGCA ATCTCTTTCG GAAATCCTTG TAATAATAAA GCCTCGTAAC CACTTAACTT ATATAATTGA TTATCCTTGA CATATAAAAT CCCGTGCCTA CCTGTTCTAA GCGTTGGTAT TTTATTTTCC ATAGAATCCG TAAAATCTGA CTGTCTCCAA TCTATTACCT GATAATTTTT CTCATATATT TGATCTAAAC TTATTCTATC CTTATTGTAT TTATTATTAA TATAATTTTC AAAAGTTTTA TTTATCAAAA ATTTATATTT ACCATCCCCA ATTAAATAAT TTGACAACTC ATTTTCATTA TTGCTTTTAG GCTCAGGAAA GACATAATCA GAATGATGTA TATCTTTTCT TATTCCAACA AAATAGATCC GTTCTCTCAT TTGTGGCGTA CCATAAAATT GACTATCTAA TACTTTATAA TACACATCAT AGTTACAACT TTCTAATATT TCTAAGATTG ATTTTAAAGT TTCACCTTGG TTATGATTTA CTAATCCCTT AACATTTTCT AAAATAAAAA ATGGTACTTT TTTCACTTTC AAAATATTGC TTAAATAATA TATTATTTGC CCTCTATTAT CTAGAAAACC ATCTCTTTTC CCAATGATAG AAAATGTTTG GCAAGGAAAT CCTGCAATCA TTAAATCAAA ATCAGGTAAA GATTTTGGAG CAAGTTGAGT TAAATCACCT AAATTACGAC TATCATTAAA AAATATTTCA TATGTTTTTG CAGGATTAAG ATCGACTTCG CTATGAGCAA TACATTCCAT CCCGTTAAAC TCTAAGCCTA ATCGCCCCCC ACCAATACCT GCACAAAAAT CAATAAACCT TAGTTTTTCC ATTAAAAATC TCCTGAACTA AAAAATAGTT TTATTTTGCA TACCTATAAT TTTTCTGAAT TACCCAAGGC TGATGTTCAA TAATAATCTC ATCTTACTTT TGAAAAGTTA CTACCCTTAT CTTTTATTTT TCTCTCAAAG AACTATTTAA TAAAATTTTA TTCGTGTAGT TTGCATAAAA TGAAATCTCT TTACTCACTA ATTTTAAACC CTAACAATAA AATCTCATCA ATAACAATTT ATACCACTCA ACTAATTCCT CAGCAATTTG CGTAATTCTC TTATCATAAA AATGAAAAAT AACTCCAGGC ATTTCACCCG AGTTATCATA AATTAACGCT TCATCACAAG ATAAAAGGGC TGTTATTGAA TTCGAGCTCG GTAGCGCGCG CTCCTCTAGA GTCGACCTGC AGGCATGCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTC