Description
Recognition site and hydrolysis position:
| CCCGC(N)4 | CCCGC(N)4↑ |
| GGGCG(N)6 | GGGCG(N)6↓ |
Source: An E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 100 | 25 | 0 | 0 | 50 | 50 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycero; Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these 95% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55°CReagents Supplied with Enzyme
10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation.
Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References:
Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova,N.I., Dedkov, V.S. Bioorg. Khim. 15: 130-132 (1989).
