SibEnzyme restriction enzymes database

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AccBS I

Enzyme name AccBS I
Prototype BsrBI
SKU SE-E007
Turbo version Available
High-concentration version Not available
Recognition site
5'… GAG▼CGG …3'
3'… CTC▲GCC …5'
Source Acinetobacter calcoaceticus BS
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75752525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Abdurashitov, M.A., Kileva, E.V., Myakisheva, T.V., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.Kh., Prikl. Biochim. Microbiol., 5(33): 556-558 (1997). M. A. Abdurashitov, E. V. Kileva, T. V. Myakisheva, V. S. Dedkov, A. V. Shevchenko, and S. Kh. Degtyarev AccBSI: A New Restriction Endonuclease from Acinetobacter calcoaceticus BS // Translated from APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol. 33, No. 5, pp. 496-498, 1997

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AccBS I

Enzyme name AccBS I
Prototype BsrBI
SKU SE-E007
Turbo version Available
High-concentration version Not available
Recognition site
5'… GAG▼CGG …3'
3'… CTC▲GCC …5'
Source Acinetobacter calcoaceticus BS
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75752525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Abdurashitov, M.A., Kileva, E.V., Myakisheva, T.V., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.Kh., Prikl. Biochim. Microbiol., 5(33): 556-558 (1997). M. A. Abdurashitov, E. V. Kileva, T. V. Myakisheva, V. S. Dedkov, A. V. Shevchenko, and S. Kh. Degtyarev AccBSI: A New Restriction Endonuclease from Acinetobacter calcoaceticus BS // Translated from APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol. 33, No. 5, pp. 496-498, 1997